We describe in detail the labelling of interleukin-2 with I ( I-IL2), its biochemical characterization, the binding assay and its use for the detection of tissues infiltrated with mononuclear cells. Human recombinant IL2 was labelled using an enzymatic method and its biochemical characterization was performed using high performance liquid chromatography (HPLC) analysis of cyanogen bromide-cleaved protein. biological and binding assays were performed on CTLL-2 cell line and on activated peripheral blood lymphocytes. studies were performed 1 h after administration of 2-3 mCi of I-IL2 in 10 newly diagnosed type 1 diabetes patients, five pre-diabetic patients, 10 Hashimoto's thyroiditis patients, 10 coeliac disease patients and 10 normal volunteers. I-IL2 scintigraphy allowed the detection and quantification of activated mononuclear cells in several affected tissues. In detail, I-IL2 accumulation was detected in the thyroid of all patients affected by Hashimoto's thyroiditis, in the bowel of all coeliac disease patients and in the pancreas of all pre-type 1 diabetic patients. By contrast, in newly diagnosed type 1 diabetics, I-IL2 scan was positive in five of the 10 studied patients. I-IL2 scintigraphy may be useful for studying autoimmune phenomena and in diagnostic protocols to evaluate the presence of other tissue involvement in patients with an organ-specific autoimmune disease.

Signore, A., Picarelli, A., Annovazzi, A., Britton, K., Grossman, A., Bonanno, E., et al. (2003). 123I-Interleukin-2: biochemical characterization and in vivo use for imaging autoimmune diseases. NUCLEAR MEDICINE COMMUNICATIONS, 24(3), 305-316 [10.1097/01.mnm.0000061052.02423.2c].

123I-Interleukin-2: biochemical characterization and in vivo use for imaging autoimmune diseases

Bonanno E;Pozzilli P.
2003-03-01

Abstract

We describe in detail the labelling of interleukin-2 with I ( I-IL2), its biochemical characterization, the binding assay and its use for the detection of tissues infiltrated with mononuclear cells. Human recombinant IL2 was labelled using an enzymatic method and its biochemical characterization was performed using high performance liquid chromatography (HPLC) analysis of cyanogen bromide-cleaved protein. biological and binding assays were performed on CTLL-2 cell line and on activated peripheral blood lymphocytes. studies were performed 1 h after administration of 2-3 mCi of I-IL2 in 10 newly diagnosed type 1 diabetes patients, five pre-diabetic patients, 10 Hashimoto's thyroiditis patients, 10 coeliac disease patients and 10 normal volunteers. I-IL2 scintigraphy allowed the detection and quantification of activated mononuclear cells in several affected tissues. In detail, I-IL2 accumulation was detected in the thyroid of all patients affected by Hashimoto's thyroiditis, in the bowel of all coeliac disease patients and in the pancreas of all pre-type 1 diabetic patients. By contrast, in newly diagnosed type 1 diabetics, I-IL2 scan was positive in five of the 10 studied patients. I-IL2 scintigraphy may be useful for studying autoimmune phenomena and in diagnostic protocols to evaluate the presence of other tissue involvement in patients with an organ-specific autoimmune disease.
mar-2003
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/36 - DIAGNOSTICA PER IMMAGINI E RADIOTERAPIA
English
Reference Values; Tomography, Emission-Computed, Single-Photon; Autoimmune Diseases; Reproducibility of Results; Humans; Tomography, X-Ray Computed; Diabetes Mellitus; Amino Acid Sequence; Child; Lymphocytes; Isotope Labeling; Lymphocyte Count; Diabetes Mellitus, Type 1; Cells, Cultured; Interleukin-2; Adult; Thyroiditis, Autoimmune; Molecular Sequence Data; Iodine Radioisotopes; Celiac Disease; Adolescent; Male; Female
Signore, A., Picarelli, A., Annovazzi, A., Britton, K., Grossman, A., Bonanno, E., et al. (2003). 123I-Interleukin-2: biochemical characterization and in vivo use for imaging autoimmune diseases. NUCLEAR MEDICINE COMMUNICATIONS, 24(3), 305-316 [10.1097/01.mnm.0000061052.02423.2c].
Signore, A; Picarelli, A; Annovazzi, A; Britton, K; Grossman, A; Bonanno, E; Maras, B; Barra, D; Pozzilli, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/194412
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