Somatostatin is a peptide hormone that exerts antisecretory and antiproliferative activities on some human tumors. The Ku70/86 heterodimer acts as regulatory subunit of the DNA dependent protein kinase and its DNA binding activity mediates DNA double strands breaks repair that is crucial to maintain the genetic integrity of the genome. The activation of the heterodimer regulates cell cycle progression and the activity of nuclear transcription factors involved in DNA replication and cell proliferation. Moreover Ku86 behaves as a receptor for the growth inhibitory tetradecapeptide, somatostatin. Herein we report that somatostatin treatment to a colon carcinoma cell line (Caco-2) inhibits cell growth and, at same time, strongly modulates the activation of Ku70/86 heterodimer and the levels of Ku86 in the nucleus by increasing its specific mRNA level. Our findings are consistent with the hypothesis that somatostatin controls cell cycle progression and DNA repair through a new signalling pathway that involves the regulation of Ku86 level and modulates the Ku70/86 activity in the nucleus.

Pucci, S., Bonanno, E., Pichiorri, F., Mazzarelli, P., Spagnoli, L. (2004). The expression and the nuclear activity of the caretaker gene ku86 are modulated by somatostatin. EUROPEAN JOURNAL OF HISTOCHEMISTRY, 48(2), 103-110.

The expression and the nuclear activity of the caretaker gene ku86 are modulated by somatostatin

Pucci S;Bonanno E;Pichiorri F;Mazzarelli P;Spagnoli LG
2004-01-01

Abstract

Somatostatin is a peptide hormone that exerts antisecretory and antiproliferative activities on some human tumors. The Ku70/86 heterodimer acts as regulatory subunit of the DNA dependent protein kinase and its DNA binding activity mediates DNA double strands breaks repair that is crucial to maintain the genetic integrity of the genome. The activation of the heterodimer regulates cell cycle progression and the activity of nuclear transcription factors involved in DNA replication and cell proliferation. Moreover Ku86 behaves as a receptor for the growth inhibitory tetradecapeptide, somatostatin. Herein we report that somatostatin treatment to a colon carcinoma cell line (Caco-2) inhibits cell growth and, at same time, strongly modulates the activation of Ku70/86 heterodimer and the levels of Ku86 in the nucleus by increasing its specific mRNA level. Our findings are consistent with the hypothesis that somatostatin controls cell cycle progression and DNA repair through a new signalling pathway that involves the regulation of Ku86 level and modulates the Ku70/86 activity in the nucleus.
2004
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/08 - Anatomia Patologica
English
Con Impact Factor ISI
RNA, Messenger; Humans; Dimerization; DNA-Binding Proteins; DNA; Cell Line, Tumor; Cell Nucleus; Immunohistochemistry; Antigens, Nuclear; Somatostatin; Cell Division
Pucci, S., Bonanno, E., Pichiorri, F., Mazzarelli, P., Spagnoli, L. (2004). The expression and the nuclear activity of the caretaker gene ku86 are modulated by somatostatin. EUROPEAN JOURNAL OF HISTOCHEMISTRY, 48(2), 103-110.
Pucci, S; Bonanno, E; Pichiorri, F; Mazzarelli, P; Spagnoli, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/194394
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