A clinical association between thyroid dysfunction and pregnancy complications has been extensively reported; however, the molecular mechanisms through which TH might regulate key events of pregnancy have not been elucidated yet. In this respect, we performed in vivo studies in MMI-induced hypothyroid pregnant mice, evaluating the effect of hypothyroidism on the number of implantation sites, developing embryos/resorptions and pups per litter, at 4.5, 10.5, 18.5 days post-coitum (dpc) and at birth. We also studied the expression of major molecules involved in implantation and placentation, such as the proteases ISPs, MMPs, TIMPs and Notch pathway-related genes. Our results demonstrate that hypothyroidism may have a dual effect on pregnancy, by initially influencing implantation and by regulating placental development at later stages of gestation. To further elucidate the role of TH in implantation, we performed in vitro studies by culturing 3.5 dpc blastocysts in the presence of TH, with or without endometrial cells used as the feeder layer, and studied their ability to undergo hatching and outgrowth. We observed that, in the presence of endometrial feeder cells, TH is able to anticipate blastocyst hatching by upregulating the expression of blastocyst-produced ISPs, and to enhance blastocyst outgrowth by upregulating endometrial ISPs and MMPs. These results clearly indicate that TH is involved in the bidirectional crosstalk between the competent blastocyst and the receptive endometrium at the time of implantation.

Piccirilli, D., Baldini, E., Massimiani, M., Camaioni, A., Salustri, A., Bernardini, R., et al. (2018). Thyroid hormone regulates protease expression and activation of Notch signaling in implantation and embryo development. JOURNAL OF ENDOCRINOLOGY, 236(1), 1-12 [10.1530/JOE-17-0436].

Thyroid hormone regulates protease expression and activation of Notch signaling in implantation and embryo development

Massimiani M.;Camaioni A.;Salustri A.;Moretti C.;Campagnolo L.
2018

Abstract

A clinical association between thyroid dysfunction and pregnancy complications has been extensively reported; however, the molecular mechanisms through which TH might regulate key events of pregnancy have not been elucidated yet. In this respect, we performed in vivo studies in MMI-induced hypothyroid pregnant mice, evaluating the effect of hypothyroidism on the number of implantation sites, developing embryos/resorptions and pups per litter, at 4.5, 10.5, 18.5 days post-coitum (dpc) and at birth. We also studied the expression of major molecules involved in implantation and placentation, such as the proteases ISPs, MMPs, TIMPs and Notch pathway-related genes. Our results demonstrate that hypothyroidism may have a dual effect on pregnancy, by initially influencing implantation and by regulating placental development at later stages of gestation. To further elucidate the role of TH in implantation, we performed in vitro studies by culturing 3.5 dpc blastocysts in the presence of TH, with or without endometrial cells used as the feeder layer, and studied their ability to undergo hatching and outgrowth. We observed that, in the presence of endometrial feeder cells, TH is able to anticipate blastocyst hatching by upregulating the expression of blastocyst-produced ISPs, and to enhance blastocyst outgrowth by upregulating endometrial ISPs and MMPs. These results clearly indicate that TH is involved in the bidirectional crosstalk between the competent blastocyst and the receptive endometrium at the time of implantation.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17
English
Con Impact Factor ISI
blastocyst; implantation; placenta; proteases; thyroid hormone; Animals; Blastocyst; Cells, Cultured; Coculture Techniques; Embryo Implantation; Embryonic Development; Endometrium; Enzyme Activation; Female; Gene Expression Regulation, Developmental; Hypothyroidism; Male; Methimazole; Mice; Peptide Hydrolases; Pregnancy; Receptors, Notch; Serine Endopeptidases; Signal Transduction; Thyroid Hormones
Piccirilli, D., Baldini, E., Massimiani, M., Camaioni, A., Salustri, A., Bernardini, R., et al. (2018). Thyroid hormone regulates protease expression and activation of Notch signaling in implantation and embryo development. JOURNAL OF ENDOCRINOLOGY, 236(1), 1-12 [10.1530/JOE-17-0436].
Piccirilli, D; Baldini, E; Massimiani, M; Camaioni, A; Salustri, A; Bernardini, R; Centanni, M; Ulisse, S; Moretti, C; Campagnolo, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/192389
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