Kaposi's sarcoma (KS) is frequently associated with human immunodeficiency virus-1 (HIV-1) infection. Supernatants from HIV-1-infected T cells carrying the CD4 antigen promote the growth of cells derived from KS lesions of AIDS patients (AIDS-KS cells), and the HIV-1 tat gene, introduced into the germ line of mice, induces skin lesions closely resembling KS. Here we report that the tat gene product (Tat) is released from both HIV-1-acutely infected H9 cells and tat-transfected COS-1 cells. These Tat-containing supernatants specifically promote growth of AIDS-KS cells which are inhibited by anti-Tat antibodies; recombinant Tat has the same growth-promoting properties. Therefore a viral regulatory gene product can be released as a biologically active protein and directly act as a growth stimulator. These and previous data indicate that extracellular Tat could be involved in the development or progression, or both, of KS in HIV-1-infected individuals.

Ensoli, B., Barillari, G., Salahuddin, S.z., Gallo, R.c., & Wong-Staal, F. (1990). Tat protein of HIV-1 stimulates growth of cells derived from Kaposi's sarcoma lesions of AIDS patients. NATURE, 345(6270), 84-86 [10.1038/345084a0].

Tat protein of HIV-1 stimulates growth of cells derived from Kaposi's sarcoma lesions of AIDS patients

BARILLARI, GIOVANNI;
1990-05-03

Abstract

Kaposi's sarcoma (KS) is frequently associated with human immunodeficiency virus-1 (HIV-1) infection. Supernatants from HIV-1-infected T cells carrying the CD4 antigen promote the growth of cells derived from KS lesions of AIDS patients (AIDS-KS cells), and the HIV-1 tat gene, introduced into the germ line of mice, induces skin lesions closely resembling KS. Here we report that the tat gene product (Tat) is released from both HIV-1-acutely infected H9 cells and tat-transfected COS-1 cells. These Tat-containing supernatants specifically promote growth of AIDS-KS cells which are inhibited by anti-Tat antibodies; recombinant Tat has the same growth-promoting properties. Therefore a viral regulatory gene product can be released as a biologically active protein and directly act as a growth stimulator. These and previous data indicate that extracellular Tat could be involved in the development or progression, or both, of KS in HIV-1-infected individuals.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/05 - Patologia Clinica
English
Con Impact Factor ISI
Acquired Immunodeficiency Syndrome; Cell Division; Cell Line; Escherichia coli; Gene Expression; Gene Products, tat; HIV-1; Humans; Immunosorbent Techniques; Recombinant Proteins; Sarcoma, Kaposi; Trans-Activators; Transfection; tat Gene Products, Human Immunodeficiency Virus
Ensoli, B., Barillari, G., Salahuddin, S.z., Gallo, R.c., & Wong-Staal, F. (1990). Tat protein of HIV-1 stimulates growth of cells derived from Kaposi's sarcoma lesions of AIDS patients. NATURE, 345(6270), 84-86 [10.1038/345084a0].
Ensoli, B; Barillari, G; Salahuddin, S; Gallo, R; Wong Staal, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/188639
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