Extracellular human immunodeficiency virus type 1 (HIV-1) Tat protein promotes growth of spindle cells derived from AIDS-associated Kaposi sarcoma (AIDS-KS), an angioproliferative disease very frequent in HIV-1-infected individuals. Normal vascular cells, progenitors of AIDS-KS cells, proliferate in response to Tat after exposure to inflammatory cytokines, whose levels are augmented in HIV-1-infected individuals and in KS lesions. Here we show that Tat also promotes AIDS-KS and normal vascular cells to migrate and to degrade the basement membrane and stimulates endothelial cell morphogenesis on a matrix substrate. These effects are obtained at picomolar concentrations of exogenous Tat and are promoted by the treatment of the cells with the same inflammatory cytokines stimulating expression of the receptors for Tat, the integrins alpha 5 beta 1 and alpha v beta 3. Thus, under specific circumstances, Tat has angiogenic properties. As Tat and its receptors are present in AIDS-KS lesions, these data may explain some of the mechanisms by which Tat can induce angiogenesis and cooperate in the development of AIDS-KS.

Albini, A., Barillari, G., Benelli, R., Gallo, R., Ensoli, B. (1995). Angiogenic properties of human immunodeficiency virus type 1 Tat protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 92(11), 4838-4842 [10.1073/pnas.92.11.4838].

Angiogenic properties of human immunodeficiency virus type 1 Tat protein

BARILLARI, GIOVANNI;
1995-05-23

Abstract

Extracellular human immunodeficiency virus type 1 (HIV-1) Tat protein promotes growth of spindle cells derived from AIDS-associated Kaposi sarcoma (AIDS-KS), an angioproliferative disease very frequent in HIV-1-infected individuals. Normal vascular cells, progenitors of AIDS-KS cells, proliferate in response to Tat after exposure to inflammatory cytokines, whose levels are augmented in HIV-1-infected individuals and in KS lesions. Here we show that Tat also promotes AIDS-KS and normal vascular cells to migrate and to degrade the basement membrane and stimulates endothelial cell morphogenesis on a matrix substrate. These effects are obtained at picomolar concentrations of exogenous Tat and are promoted by the treatment of the cells with the same inflammatory cytokines stimulating expression of the receptors for Tat, the integrins alpha 5 beta 1 and alpha v beta 3. Thus, under specific circumstances, Tat has angiogenic properties. As Tat and its receptors are present in AIDS-KS lesions, these data may explain some of the mechanisms by which Tat can induce angiogenesis and cooperate in the development of AIDS-KS.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/05 - Patologia Clinica
English
Con Impact Factor ISI
Acquired Immunodeficiency Syndrome; Aorta, Abdominal; CD4-Positive T-Lymphocytes; Cell Division; Cell Movement; Cells, Cultured; Culture Media, Conditioned; Cytokines; Endothelium, Vascular; Gene Products, tat; Granulocyte-Macrophage Colony-Stimulating Factor; HIV-1; Humans; Interferon-gamma; Interleukin-1; Interleukin-2; Interleukin-6; Leukemia, T-Cell; Lymphotoxin-alpha; Morphogenesis; Muscle, Smooth, Vascular; Sarcoma, Kaposi; Tumor Necrosis Factor-alpha; Umbilical Veins; tat Gene Products, Human Immunodeficiency Virus; Neovascularization, Pathologic
Albini, A., Barillari, G., Benelli, R., Gallo, R., Ensoli, B. (1995). Angiogenic properties of human immunodeficiency virus type 1 Tat protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 92(11), 4838-4842 [10.1073/pnas.92.11.4838].
Albini, A; Barillari, G; Benelli, R; Gallo, R; Ensoli, B
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/188548
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