Naegleria spp. are free-living amoebae belonging to the family Vahlkampfiidae, in the class Heterolobosea. Among the recognized species, Naegleria fowleri causes primary amoebic meningoencephalitis (PAM), while two other species, Naegleria australiensis and Naegleria italica, have been reported as pathogenic in experimental animals. Due to the thermotolerance properties of some species, geothermal water sources including hot springs represent suitable habitats for their proliferation. The main aim of this study was a year-round sampling in two geothermal springs in Central Italy, to investigate the presence of Naegleria spp. using PCR/DNA sequencing based methods. The affinities between the sequences generated here and others reported in the literature were explored by using POY, which implements the concept of dynamic homology. Naegleria australiensis, Naegleria italica, and Naegleria lovaniensis, plus an unassigned Naegleria spp. were detected. Indels in the rDNA ITS1 and ITS2 turned out to be critical to distinguish the three species and confirmed their phylogenetic relationships. This is the first molecular report on the Naegleria spp. occurrence in geothermal waters in Central Italy, coupled with a fine genetic characterization.

MONTALBANO DI FILIPPO, M., Novelletto, A., DI CAVE, D., Berrilli, F. (2017). Identification and phylogenetic position of Naegleria spp. from geothermal springs in Italy. EXPERIMENTAL PARASITOLOGY, 183, 143-149 [10.1016/j.exppara.2017.08.008].

Identification and phylogenetic position of Naegleria spp. from geothermal springs in Italy

MONTALBANO DI FILIPPO, MARGHERITA;NOVELLETTO, ANDREA;DI CAVE, DAVID;BERRILLI, FEDERICA
2017

Abstract

Naegleria spp. are free-living amoebae belonging to the family Vahlkampfiidae, in the class Heterolobosea. Among the recognized species, Naegleria fowleri causes primary amoebic meningoencephalitis (PAM), while two other species, Naegleria australiensis and Naegleria italica, have been reported as pathogenic in experimental animals. Due to the thermotolerance properties of some species, geothermal water sources including hot springs represent suitable habitats for their proliferation. The main aim of this study was a year-round sampling in two geothermal springs in Central Italy, to investigate the presence of Naegleria spp. using PCR/DNA sequencing based methods. The affinities between the sequences generated here and others reported in the literature were explored by using POY, which implements the concept of dynamic homology. Naegleria australiensis, Naegleria italica, and Naegleria lovaniensis, plus an unassigned Naegleria spp. were detected. Indels in the rDNA ITS1 and ITS2 turned out to be critical to distinguish the three species and confirmed their phylogenetic relationships. This is the first molecular report on the Naegleria spp. occurrence in geothermal waters in Central Italy, coupled with a fine genetic characterization.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
Settore BIO/18 - Genetica
English
Free living amoebae; Geothermal water; Molecular taxonomy; Naegleria spp.; Prevalence; rDNA diversity
MONTALBANO DI FILIPPO, M., Novelletto, A., DI CAVE, D., Berrilli, F. (2017). Identification and phylogenetic position of Naegleria spp. from geothermal springs in Italy. EXPERIMENTAL PARASITOLOGY, 183, 143-149 [10.1016/j.exppara.2017.08.008].
MONTALBANO DI FILIPPO, M; Novelletto, A; DI CAVE, D; Berrilli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/187674
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