In the present work the induction of micronuclei in erythrocytes of Cyprinus carpio treated with X-rays and colchicine is studied for the evaluation of mutagenic effects of both clastogenic and mitoclastic (spindle poisoning) agents in this system. Three different experiments were performed treating groups of laboratory-reproduced animals with (1) single doses of X-rays (0.1, 0.5 and 2Gy); (2) a single i.p. injection of colchicine at the concentrations: 1.6x10(-2), 8x10(-2), 0.4 and 2mg/kg b.w. so as to mimic an acute exposure to the agent and (3) six repeated i.p. injections of the first three concentrations of colchicine, over a period of 18 days, so as to mimic a chronic exposure. Repeated blood samplings were performed by cardiac puncture over a period of about 2 months after the treatment and micronucleus frequencies were determined at multiple times on the same individuals after mutagen exposure. A dose-dependent increase in the micronucleus frequency was observed in irradiated fish and a peak value detected at 21 days. Slight increases of micronucleus frequencies were also observed in both colchicine experiments only for the highest concentrations at the earliest sampling time. Higher concentrations of colchicine clearly showed a lethal effect. According to the present data the micronucleus frequency induced by the highest colchicine dose is comparable to that observed after 0.1Gy of X-ray irradiation.

Gustavino, B., Scornajenghi, K.a., Minissi, S., & Ciccotti, E. (2001). Micronuclei induced in erythrocytes of Cyprinus carpio (teleostei, pisces) by X-rays and colchicine. MUTATION RESEARCH, 494(1-2), 151-159 [10.1016/S1383-5718(01)00191-7].

Micronuclei induced in erythrocytes of Cyprinus carpio (teleostei, pisces) by X-rays and colchicine

GUSTAVINO, BIANCA;CICCOTTI, ELEONORA
2001-07-25

Abstract

In the present work the induction of micronuclei in erythrocytes of Cyprinus carpio treated with X-rays and colchicine is studied for the evaluation of mutagenic effects of both clastogenic and mitoclastic (spindle poisoning) agents in this system. Three different experiments were performed treating groups of laboratory-reproduced animals with (1) single doses of X-rays (0.1, 0.5 and 2Gy); (2) a single i.p. injection of colchicine at the concentrations: 1.6x10(-2), 8x10(-2), 0.4 and 2mg/kg b.w. so as to mimic an acute exposure to the agent and (3) six repeated i.p. injections of the first three concentrations of colchicine, over a period of 18 days, so as to mimic a chronic exposure. Repeated blood samplings were performed by cardiac puncture over a period of about 2 months after the treatment and micronucleus frequencies were determined at multiple times on the same individuals after mutagen exposure. A dose-dependent increase in the micronucleus frequency was observed in irradiated fish and a peak value detected at 21 days. Slight increases of micronucleus frequencies were also observed in both colchicine experiments only for the highest concentrations at the earliest sampling time. Higher concentrations of colchicine clearly showed a lethal effect. According to the present data the micronucleus frequency induced by the highest colchicine dose is comparable to that observed after 0.1Gy of X-ray irradiation.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/07
English
Con Impact Factor ISI
Animals; Colchicine; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Environmental Monitoring; Erythrocytes; Micronucleus Tests; Mutagens; Whole-Body Irradiation; X-Rays; Carps
WOS KeyWords Plus:TROUT ONCORHYNCHUS-MYKISS; LONG-ISLAND-SOUND; PERIPHERAL ERYTHROCYTES; ETHYL METHANESULFONATE; NUCLEAR ABNORMALITIES; EASTERN MUDMINNOW; UMBRA-PYGMAEA; COMET ASSAY; RIVER WATER; FRESH-WATER
Scopus Indexed keywords EMTREE drug terms: clastogen, colchicine EMTREE medical terms: animal, experiment article, blood sampling, carp, controlled study, dose response, drug exposure, erythrocyte, long term exposure, micronucleus test, mitosis inhibition, mutagenicity, nonhuman priority journal, radiation dose, toxicity testing, X ray MeSH: Animals, Carps, Colchicine, Dose-Response Relationship, Drug, Dose-Response Relationship, Radiation,Environmental Monitoring, Erythrocytes Micronucleus Tests, Mutagens, Whole-Body Irradiation, X-Rays
Research Areas:Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology Web of Science Categories:Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology Metrics in Scopus 41 Citations 75th Percentile 0.74 Field-Weighted Citation Impact 29 Mendeley Readers 94th Percentile WOS Metrics All Times Cited Counts 38 in All Databases 38 in Web of Science Core Collection 36 in BIOSIS Citation Index JOURNAL INFORMATION Mutation Research - Genetic Toxicology and Environmental Mutagenesis Journal Metrics CiteScore: 2.25 More about CiteScore Impact Factor: 2.133 5-Year Impact Factor: 2.528 Source Normalized Impact per Paper (SNIP): 0.937 SCImago Journal Rank (SJR): 0.924
Gustavino, B., Scornajenghi, K.a., Minissi, S., & Ciccotti, E. (2001). Micronuclei induced in erythrocytes of Cyprinus carpio (teleostei, pisces) by X-rays and colchicine. MUTATION RESEARCH, 494(1-2), 151-159 [10.1016/S1383-5718(01)00191-7].
Gustavino, B; Scornajenghi, K; Minissi, S; Ciccotti, E
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/186705
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