Ammodytin L (AMDL) is a myotoxic phospholipase-like protein from the venom of Vipera ammodytes with a serine in position 49 instead of an aspartate, therefore this toxin is devoid of phospholipase activity, and the membrane-damaging effect does not involve any step of phospholipase activity. The aim of the present study was to analyze the effect of AMDL on L-6 cells from rat skeletal muscle to investigate its mechanism of action and the role of calcium ions in its muscle-damaging activity. Our data indicate that the effect of ammodytin L is strongly dependent on the degree of cell differentiation. Low doses of myotoxin gave rise to a marked release of creatine kinase in myotubes differentiated from L-6 myoblasts and the presence of calcium ions plays a role in the cytotoxic effect. The presence of EGTA in the incubation buffer reduced by 50% the release of creatine kinase. No membrane damage was observed in myoblasts, but there was a significant increase of intracellular calcium concentration measured with Fura-2. A non-specific membrane effect of AMDL was ruled out using platelets as reference cells: no platelet aggregation pattern and no increase in intracellular calcium were observed.
Incerpi, S., de Vito, P., Luly, P., & Rufini, S. (1995). Effect of ammodytin L from Vipera ammodytes on L-6 cells from rat skeletal muscle. BIOCHIMICA ET BIOPHYSICA ACTA, 1268(2), 137-142 [10.1016/0167-4889(95)00053-U].
|Tipologia:||Articolo su rivista|
|Citazione:||Incerpi, S., de Vito, P., Luly, P., & Rufini, S. (1995). Effect of ammodytin L from Vipera ammodytes on L-6 cells from rat skeletal muscle. BIOCHIMICA ET BIOPHYSICA ACTA, 1268(2), 137-142 [10.1016/0167-4889(95)00053-U].|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/09|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0167-4889(95)00053-U|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||31-ago-1995|
|Titolo:||Effect of ammodytin L from Vipera ammodytes on L-6 cells from rat skeletal muscle|
|Autori:||Incerpi, S; de Vito, P; Luly, P; Rufini, S|
|Appare nelle tipologie:||01 - Articolo su rivista|