Activated single wall carbon nanotubes have been used for biomedical purposes as carriers for drugs, peptides, proteins and nucleic acids. A large volume of data speaks to their suitability to act as a carrier. The ability of two differently activated SWNTs (with carboxyl groups or with carboxyl groups and polyethylenimine (PEI)) to form a complex with the hepatitis A virus was evaluated. Both types of activations permitted the formation of a virus-SWNT complex. However, their patterns were different. The carboxyl-activated nanotubes had a somewhat low adsorptive capacity that was related inversely to the concentrations of the SWNTs and viruses. Statistical analysis, using the chi(2)-test, showed no significant differences between the SWNT-PEI ratios of 1:2.5, 1:1 and 1:0.5. The addiction of PEI improved the adsorption, probably because of the electropositive charge of the molecule. Adsorption was optimal between 100 microg and 10 ng with a SWNTs-PEI weight ratio of 1:0.2 up to an inoculum of 10(5) genome equivalents of hepatitis A virus. Reducing or increasing this weight ratio reduced the adsorptive capacity of the PEI, and this adsorption activity was time and contact-dependent. Thus, SWNTs coated with PEI are able to complex with viruses, and they might be used in the future to transfect non-permissive cell lines.

Petrinca, A., Donia, D.t., Cicchetti, R., Valentini, F., Argentin, G., Carbone, M., et al. (2010). Interaction between single wall carbon nanotubes and a human enteric virus. JOURNAL OF VIROLOGICAL METHODS, 168(1-2), 1-5 [10.1016/j.jviromet.2010.03.029].

Interaction between single wall carbon nanotubes and a human enteric virus

DONIA, DOMENICA TOMMASA;CICCHETTI, ROSADELE;VALENTINI, FEDERICA;ARGENTIN, GABRIELLA;CARBONE, MARILENA;PIETROIUSTI, ANTONIO;MAGRINI, ANDREA;PALLESCHI, GIUSEPPE;DIVIZIA, MAURIZIO
2010-09-01

Abstract

Activated single wall carbon nanotubes have been used for biomedical purposes as carriers for drugs, peptides, proteins and nucleic acids. A large volume of data speaks to their suitability to act as a carrier. The ability of two differently activated SWNTs (with carboxyl groups or with carboxyl groups and polyethylenimine (PEI)) to form a complex with the hepatitis A virus was evaluated. Both types of activations permitted the formation of a virus-SWNT complex. However, their patterns were different. The carboxyl-activated nanotubes had a somewhat low adsorptive capacity that was related inversely to the concentrations of the SWNTs and viruses. Statistical analysis, using the chi(2)-test, showed no significant differences between the SWNT-PEI ratios of 1:2.5, 1:1 and 1:0.5. The addiction of PEI improved the adsorption, probably because of the electropositive charge of the molecule. Adsorption was optimal between 100 microg and 10 ng with a SWNTs-PEI weight ratio of 1:0.2 up to an inoculum of 10(5) genome equivalents of hepatitis A virus. Reducing or increasing this weight ratio reduced the adsorptive capacity of the PEI, and this adsorption activity was time and contact-dependent. Thus, SWNTs coated with PEI are able to complex with viruses, and they might be used in the future to transfect non-permissive cell lines.
set-2010
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/44 - MEDICINA DEL LAVORO
Settore MED/42 - IGIENE GENERALE E APPLICATA
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
Nanotubes, Carbon; Hepatitis A virus; Static Electricity; Virus Attachment; Electricity
Petrinca, A., Donia, D.t., Cicchetti, R., Valentini, F., Argentin, G., Carbone, M., et al. (2010). Interaction between single wall carbon nanotubes and a human enteric virus. JOURNAL OF VIROLOGICAL METHODS, 168(1-2), 1-5 [10.1016/j.jviromet.2010.03.029].
Petrinca, A; Donia, Dt; Cicchetti, R; Valentini, F; Argentin, G; Carbone, M; Pietroiusti, A; Magrini, A; Palleschi, G; Divizia, M
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/18018
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