Here we report the rational design of a synthetic molecular nanodevice that is directly inspired from hemoglobin, a highly evolved protein whose oxygen-carrying activity is finely regulated by a sophisticated network of control mechanisms. Inspired by the impressive performance of hemoglobin we have designed and engineered in vitro a synthetic DNA-based nanodevice containing up to four interacting binding sites that, like hemoglobin, can load and release a cargo over narrow concentration ranges, and whose affinity can be finely controlled via both allosteric effectors and environmental cues like pH and temperature. As the first example of a synthetic DNA nanodevice that undergoes a complex network of nature-inspired control mechanisms, this represents an important step toward the use of similar nanodevices for diagnostic and drug-delivery applications.

Mariottini, D., Idili, A., Vallée Bélisle, A., Plaxco, K., & Ricci, F. (2017). A DNA Nanodevice that loads and releases a cargo with hemoglobin-like allosteric control and cooperativity. NANO LETTERS, 17(5), 3225-3230 [10.1021/acs.nanolett.7b00814].

A DNA Nanodevice that loads and releases a cargo with hemoglobin-like allosteric control and cooperativity

MARIOTTINI, DAVIDE;IDILI, ANDREA;RICCI, FRANCESCO
2017

Abstract

Here we report the rational design of a synthetic molecular nanodevice that is directly inspired from hemoglobin, a highly evolved protein whose oxygen-carrying activity is finely regulated by a sophisticated network of control mechanisms. Inspired by the impressive performance of hemoglobin we have designed and engineered in vitro a synthetic DNA-based nanodevice containing up to four interacting binding sites that, like hemoglobin, can load and release a cargo over narrow concentration ranges, and whose affinity can be finely controlled via both allosteric effectors and environmental cues like pH and temperature. As the first example of a synthetic DNA nanodevice that undergoes a complex network of nature-inspired control mechanisms, this represents an important step toward the use of similar nanodevices for diagnostic and drug-delivery applications.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - Chimica Analitica
English
cooperativity; DNA nanomachines; DNA nanoswitches; DNA nanotechnology; molecular devices;
DNA nanotechnology, molecular devices, DNA nanoswitches, DNA nanomachines, cooperativity
Mariottini, D., Idili, A., Vallée Bélisle, A., Plaxco, K., & Ricci, F. (2017). A DNA Nanodevice that loads and releases a cargo with hemoglobin-like allosteric control and cooperativity. NANO LETTERS, 17(5), 3225-3230 [10.1021/acs.nanolett.7b00814].
Mariottini, D; Idili, A; Vallée Bélisle, A; Plaxco, K; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/179704
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