Cellular senescence is a stable cell cycle arrest that is the causative process of aging. The PI3K/AKT/mTOR pathway is implicated in the control of cellular senescence and inhibitors of this pathway have been successfully used for life span prolongation experiments in mammals. PTEN is the major regulator of the PI3K/AKT/mTOR pathway and loss of PTEN promotes a senescence response termed PICS. Here we report a novel-screening assay, for the identification of compounds that block different types of senescence response. By testing a library of more than 3000 natural and chemical compounds in PTEN deficient cells we have found that an extract from Salvia haenkei (SH), a native plant of Bolivia is a potent inhibitor of PICS. SH also decreases replicative and UV-mediated senescence in human primary fibroblasts and in a model of in vitro reconstructed human epidermis. Mechanistically, SH treatment affects senescence driven by UV by interfering with IL1-α signalling. Pre-clinical and clinical testing of this extract by performing toxicity and irritability evaluation in vitro also demonstrate the safety of SH extract for clinical use as anti-aging skin treatment.
Matic, I., Revandkar, A., Chen, J., Bisio, A., Dall'Acqua, S., Cocetta, V., et al. (2016). Identification of Salvia haenkei as gerosuppressant agent by using an integrated senescence-screening assay. AGING, 8(12), 3223-3240 [10.18632/aging.101076].
|Tipologia:||Articolo su rivista|
|Citazione:||Matic, I., Revandkar, A., Chen, J., Bisio, A., Dall'Acqua, S., Cocetta, V., et al. (2016). Identification of Salvia haenkei as gerosuppressant agent by using an integrated senescence-screening assay. AGING, 8(12), 3223-3240 [10.18632/aging.101076].|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.18632/aging.101076|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2016|
|Titolo:||Identification of Salvia haenkei as gerosuppressant agent by using an integrated senescence-screening assay|
|Autori:||Matic, I; Revandkar, A; Chen, J; Bisio, A; Dall'Acqua, S; Cocetta, V; Brun, P; Mancino, G; Milanese, M; Mattei, M; Montopoli, M; Alimonti, A|
|Appare nelle tipologie:||01 - Articolo su rivista|