Gammadelta T lymphocytes recognize nonpeptidic microbial antigens without MHC restriction and display both lytic and proliferative responses to human immunodeficiency virus (HIV)-infected cells. This innate recognition involves both T Cell Receptor (TCR) and NK-receptor mediated signalling through non-peptidic metabolites and HLA class I down-regulation. We observed that HLA-masking and nonpeptidic phosphoantigens induce the expression of CD25 and CD69 activation markers on the surface of gammadelta T cells. Interestingly, CD94+ cell depletion by magnetic beads showed that the expression of this antigen is essential for Vdelta2 T cell activation by HLA-masking. Moreover, both phosphoantigen-stimulation and in vitro HIV infection resulted in marked Vgamma9Vdelta2 T cell expansion, whereas HLA-masking was unable to induce proliferative responses. Finally, we observed a relevant hyporesponsiveness to non-peptidic antigens in HIV-infected persons and in cord blood cells from healthy donors when compared to adult PBMC from uninfected donors. Altogether, the reduced ability to naturally recognize the infected cells may contribute to HIV-disease progression and may facilitate maternal transmission of HIV infections.

Montesano, C., Gioia, C., Martini, F., Agrati, C., Cairo, C., Pucillo, L., et al. (2001). Antiviral activity and anergy of gammadeltaT lymphocytes in cord blood and immuno-compromised host. JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 15(3), 257-264.

Antiviral activity and anergy of gammadeltaT lymphocytes in cord blood and immuno-compromised host

MONTESANO, CARLA;COLIZZI, VITTORIO;
2001-07-01

Abstract

Gammadelta T lymphocytes recognize nonpeptidic microbial antigens without MHC restriction and display both lytic and proliferative responses to human immunodeficiency virus (HIV)-infected cells. This innate recognition involves both T Cell Receptor (TCR) and NK-receptor mediated signalling through non-peptidic metabolites and HLA class I down-regulation. We observed that HLA-masking and nonpeptidic phosphoantigens induce the expression of CD25 and CD69 activation markers on the surface of gammadelta T cells. Interestingly, CD94+ cell depletion by magnetic beads showed that the expression of this antigen is essential for Vdelta2 T cell activation by HLA-masking. Moreover, both phosphoantigen-stimulation and in vitro HIV infection resulted in marked Vgamma9Vdelta2 T cell expansion, whereas HLA-masking was unable to induce proliferative responses. Finally, we observed a relevant hyporesponsiveness to non-peptidic antigens in HIV-infected persons and in cord blood cells from healthy donors when compared to adult PBMC from uninfected donors. Altogether, the reduced ability to naturally recognize the infected cells may contribute to HIV-disease progression and may facilitate maternal transmission of HIV infections.
lug-2001
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - PATOLOGIA GENERALE
English
Receptors, Antigen, T-Cell, gamma-delta; Antigens, Differentiation, T-Lymphocyte; HIV Infections; T-Lymphocytes; Interferon-gamma; Antigens, CD; Humans; Tumor Necrosis Factor-alpha; HLA Antigens; Fetal Blood; Receptors, Interleukin-2; Immune Tolerance; Adult; Infant, Newborn; Lectins, C-Type
Montesano, C., Gioia, C., Martini, F., Agrati, C., Cairo, C., Pucillo, L., et al. (2001). Antiviral activity and anergy of gammadeltaT lymphocytes in cord blood and immuno-compromised host. JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 15(3), 257-264.
Montesano, C; Gioia, C; Martini, F; Agrati, C; Cairo, C; Pucillo, L; Colizzi, V; Poccia, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/17263
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