Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 degrees C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 degrees C and less than 0.02% at 45 degrees C. When protein synthesis is inhibited during heat shock the fraction of surviving cells increases to 23% at 43 degrees C and 0.9% at 45 degrees C. This suggest that part of the cells that die upon heat shock are not heavily damaged and would have survived in the presence of a block in protein synthesis.

Ghibelli, L., Nosseri, C., Oliverio, S., Piacentini, M., & Autuori, F. (1992). Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis. EXPERIMENTAL CELL RESEARCH, 201(2), 436-443.

Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis

GHIBELLI, LINA;OLIVERIO, SERAFINA;PIACENTINI, MAURO;AUTUORI, FRANCESCO
1992-08

Abstract

Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 degrees C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 degrees C and less than 0.02% at 45 degrees C. When protein synthesis is inhibited during heat shock the fraction of surviving cells increases to 23% at 43 degrees C and 0.9% at 45 degrees C. This suggest that part of the cells that die upon heat shock are not heavily damaged and would have survived in the presence of a block in protein synthesis.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/13
English
Animals; Antibodies; Cell Death; Cycloheximide; Enzyme Induction; Hot Temperature; L Cells (Cell Line); Mice; Transglutaminases
Ghibelli, L., Nosseri, C., Oliverio, S., Piacentini, M., & Autuori, F. (1992). Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis. EXPERIMENTAL CELL RESEARCH, 201(2), 436-443.
Ghibelli, L; Nosseri, C; Oliverio, S; Piacentini, M; Autuori, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/170681
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