Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.

De Liberato, C., Berrilli, F., Marangi, M., Santoro, M., Trogu, T., Putignani, L., et al. (2015). Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois. PARASITES & VECTORS, 8(1), 650 [10.1186/s13071-015-1243-1].

Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois

BERRILLI, FEDERICA;
2015

Abstract

Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
English
Con Impact Factor ISI
Giardia duodenalis, Rupicapra rupicapra rupicapra, Rupicapra pyrenaica ornata, IF, qPCR, end-point-PCR
Supported by MIUR-Relevant Italian Project (PRIN) grant number: 2010P7LFW4 (2013–2016)
De Liberato, C., Berrilli, F., Marangi, M., Santoro, M., Trogu, T., Putignani, L., et al. (2015). Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois. PARASITES & VECTORS, 8(1), 650 [10.1186/s13071-015-1243-1].
De Liberato, C; Berrilli, F; Marangi, M; Santoro, M; Trogu, T; Putignani, L; Lanfranchi, P; Ferretti, F; D'Amelio, S; Giangaspero, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/170207
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