We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy.
Colosimo, A., Serafino, A., Sangiuolo, F.C., Di Sario, S., Bruscia, E., Amicucci, P., et al. (1999). Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes. BIOCHIMICA ET BIOPHYSICA ACTA, 1419(2), 186-194 [10.1016/S0005-2736(99)00067-X].
Tipologia: | Articolo su rivista | |
Citazione: | Colosimo, A., Serafino, A., Sangiuolo, F.C., Di Sario, S., Bruscia, E., Amicucci, P., et al. (1999). Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes. BIOCHIMICA ET BIOPHYSICA ACTA, 1419(2), 186-194 [10.1016/S0005-2736(99)00067-X]. | |
IF: | Con Impact Factor ISI | |
Lingua: | English | |
Settore Scientifico Disciplinare: | Settore MED/03 - Genetica Medica | |
Revisione (peer review): | Esperti anonimi | |
Tipo: | Articolo | |
Rilevanza: | Rilevanza internazionale | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0005-2736(99)00067-X | |
Stato di pubblicazione: | Pubblicato | |
Data di pubblicazione: | 15-lug-1999 | |
Titolo: | Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes | |
Autori: | ||
Autori: | Colosimo, A; Serafino, A; Sangiuolo, FC; Di Sario, S; Bruscia, E; Amicucci, P; Novelli, G; Dallapiccola, B; Mossa, G | |
Appare nelle tipologie: | 01 - Articolo su rivista |