We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy.

Colosimo, A., Serafino, A., Sangiuolo, F.c., Di Sario, S., Bruscia, E., Amicucci, P., et al. (1999). Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes. BIOCHIMICA ET BIOPHYSICA ACTA, 1419(2), 186-194 [10.1016/S0005-2736(99)00067-X].

Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes

SANGIUOLO, FEDERICA CARLA;NOVELLI, GIUSEPPE;
1999-07-15

Abstract

We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy.
15-lug-1999
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/03 - GENETICA MEDICA
English
Con Impact Factor ISI
Cell Line, Transformed; Cholesterol; DNA; Epithelial Cells; Fluorescent Dyes; Genetic Therapy; Humans; Liposomes; Microscopy, Confocal; Microscopy, Electron; Phosphatidylethanolamines; Trachea; Transfection; Organic Chemicals
Colosimo, A., Serafino, A., Sangiuolo, F.c., Di Sario, S., Bruscia, E., Amicucci, P., et al. (1999). Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes. BIOCHIMICA ET BIOPHYSICA ACTA, 1419(2), 186-194 [10.1016/S0005-2736(99)00067-X].
Colosimo, A; Serafino, A; Sangiuolo, Fc; Di Sario, S; Bruscia, E; Amicucci, P; Novelli, G; Dallapiccola, B; Mossa, G
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/166514
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