SFHR (small fragment homologous replacement)-mediated targeting is a process that has been used to correct specific mutations in mammalian cells. This process involves both chemical and cellular factors that are not yet defined. To evaluate potential of this technique for gene therapy it is necessary to characterize gene transfer efficacy in terms of the transfection vehicle, the genetic target, and the cellular processing of the DNA and DNA-vehicle complex.

Sangiuolo, F., Bruscia, E., Serafino, A., Nardone, A.m., Bonifazi, E., Lais, M., et al. (2002). In vitro correction of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique. BMC MEDICAL GENETICS, 3, 8 [10.1186/1471-2350-3-8].

In vitro correction of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique

SANGIUOLO, FEDERICA CARLA;BONIFAZI, EMANUELA;NOVELLI, GIUSEPPE
2002-09-23

Abstract

SFHR (small fragment homologous replacement)-mediated targeting is a process that has been used to correct specific mutations in mammalian cells. This process involves both chemical and cellular factors that are not yet defined. To evaluate potential of this technique for gene therapy it is necessary to characterize gene transfer efficacy in terms of the transfection vehicle, the genetic target, and the cellular processing of the DNA and DNA-vehicle complex.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/03 - Genetica Medica
English
Con Impact Factor ISI
Sangiuolo, F., Bruscia, E., Serafino, A., Nardone, A.m., Bonifazi, E., Lais, M., et al. (2002). In vitro correction of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique. BMC MEDICAL GENETICS, 3, 8 [10.1186/1471-2350-3-8].
Sangiuolo, Fc; Bruscia, E; Serafino, A; Nardone, A; Bonifazi, E; Lais, M; Gruenert, D; Novelli, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/166467
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