The objectives of the present study were to assess the occurrence of human adenoviruses (HAdVs) in paediatric patients with gastroenteritis in Albania and to characterize HAdV strains. Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene. Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains. Adenovirus DNA was detected in 33/142 samples (23.2%); 14 belonged to species F (13 HAdV-41 and 1 HAdV-40), 13 to species C (1 HAdV-1, 8 HAdV-2, and 4 HAdV-5), 5 to species B (HAdV-3), and 1 to species A (HAdV-12). Rotavirus coinfection was present in 9/33 (27.2%) positive samples. In the remaining 24 positive samples (12 enteric--F species; 12 nonenteric--A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization. This is the first study investigating the presence of human adenoviruses (species A-G) as etiologic agents of viral gastroenteritis in children in Albania.

La Rosa, G., Della Libera, S., Petricca, S., Iaconelli, M., Donia, D.T., Saccucci, P., et al. (2015). Genetic diversity of human adenovirus in children with acute gastroenteritis, Albania, 2013-2015. BIOMED RESEARCH INTERNATIONAL, 2015, 1-7 [10.1155/2015/142912].

Genetic diversity of human adenovirus in children with acute gastroenteritis, Albania, 2013-2015

DONIA, DOMENICA TOMMASA;SACCUCCI, PATRIZIA;DIVIZIA, MAURIZIO
2015

Abstract

The objectives of the present study were to assess the occurrence of human adenoviruses (HAdVs) in paediatric patients with gastroenteritis in Albania and to characterize HAdV strains. Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene. Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains. Adenovirus DNA was detected in 33/142 samples (23.2%); 14 belonged to species F (13 HAdV-41 and 1 HAdV-40), 13 to species C (1 HAdV-1, 8 HAdV-2, and 4 HAdV-5), 5 to species B (HAdV-3), and 1 to species A (HAdV-12). Rotavirus coinfection was present in 9/33 (27.2%) positive samples. In the remaining 24 positive samples (12 enteric--F species; 12 nonenteric--A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization. This is the first study investigating the presence of human adenoviruses (species A-G) as etiologic agents of viral gastroenteritis in children in Albania.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/42 - Igiene Generale e Applicata
English
Con Impact Factor ISI
adenoviridae; Albania; child; child, preschool; face; female; gastroenteritis; genetic variation; humans; infant; male; species specificity; phylogeny
La Rosa, G., Della Libera, S., Petricca, S., Iaconelli, M., Donia, D.T., Saccucci, P., et al. (2015). Genetic diversity of human adenovirus in children with acute gastroenteritis, Albania, 2013-2015. BIOMED RESEARCH INTERNATIONAL, 2015, 1-7 [10.1155/2015/142912].
La Rosa, G; Della Libera, S; Petricca, S; Iaconelli, M; Donia, Dt; Saccucci, P; Cenko, F; Xhelilaj, G; Divizia, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/165742
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