Objectives: To investigate the ability of synovial fluid from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) to modulate cell-surface phenotype, function and viability of monocytes. Methods: Monocytes from healthy donors were incubated with synovial fluid from patients with RA or OA. These were then cultured with autologous healthy CD4+ T-cells. Immunoglobulin-like transcript 4 (ILT4) and CD86 were evaluated on stimulated monocytes and CD4+ T-cells via fluorescence activated cell sorting. Results: Monocytes incubated with synovial fluid from patients with RA (SF-RA; n = 12) had significantly lower ILT4 and higher CD86 levels than those incubated with synovial fluid from patients with OA (SF-OA; n = 12) or medium alone. In patients with RA, there was a significant negative correlation between ILT4 and disease activity score (DAS; r = −0.699), and a positive correlation between CD86 and DAS (r = 0.626). T-cells costimulated with monocytes cultured with SF-RA produced significantly more interferon-γ and tumour necrosis factor-α than those costimulated with monocytes cultured with SF-OA or controls. Conclusions: Soluble mediators in SF-RA could contribute to modulating inflammation and local effectiveness of the immune response.

Chimenti, M.s., Conigliaro, P., Triggianese, P., Baffari, E., Squillaci, E., Bolacchi, F., et al. (2016). Synovial fluid from patients with rheumatoid arthritis modulates monocyte cell-surface phenotype. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 44(1_suppl), 15-21 [10.1177/0300060515593231].

Synovial fluid from patients with rheumatoid arthritis modulates monocyte cell-surface phenotype

CHIMENTI, MARIA SOLE;CONIGLIARO, PAOLA;TRIGGIANESE, PAOLA;BAFFARI, ELEONORA;SQUILLACI, ETTORE;BOLACCHI, FRANCESCA;PERRICONE, ROBERTO;BERGAMINI, ALBERTO
2016-01-01

Abstract

Objectives: To investigate the ability of synovial fluid from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) to modulate cell-surface phenotype, function and viability of monocytes. Methods: Monocytes from healthy donors were incubated with synovial fluid from patients with RA or OA. These were then cultured with autologous healthy CD4+ T-cells. Immunoglobulin-like transcript 4 (ILT4) and CD86 were evaluated on stimulated monocytes and CD4+ T-cells via fluorescence activated cell sorting. Results: Monocytes incubated with synovial fluid from patients with RA (SF-RA; n = 12) had significantly lower ILT4 and higher CD86 levels than those incubated with synovial fluid from patients with OA (SF-OA; n = 12) or medium alone. In patients with RA, there was a significant negative correlation between ILT4 and disease activity score (DAS; r = −0.699), and a positive correlation between CD86 and DAS (r = 0.626). T-cells costimulated with monocytes cultured with SF-RA produced significantly more interferon-γ and tumour necrosis factor-α than those costimulated with monocytes cultured with SF-OA or controls. Conclusions: Soluble mediators in SF-RA could contribute to modulating inflammation and local effectiveness of the immune response.
2016
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/36 - DIAGNOSTICA PER IMMAGINI E RADIOTERAPIA
English
Con Impact Factor ISI
Anti-tumour necrosis factor- α; Immunoglobulin-like transcript 4 (ILT4); inflammation; innate immunity; rheumatoid arthritis; TNF-α; Medicine (all); Biochemistry; Cell Biology; Biochemistry (medical)
http://imr.sagepub.com/content/by/year
Chimenti, M.s., Conigliaro, P., Triggianese, P., Baffari, E., Squillaci, E., Bolacchi, F., et al. (2016). Synovial fluid from patients with rheumatoid arthritis modulates monocyte cell-surface phenotype. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 44(1_suppl), 15-21 [10.1177/0300060515593231].
Chimenti, Ms; Conigliaro, P; Triggianese, P; Baffari, E; Squillaci, E; Bolacchi, F; Perricone, R; Bergamini, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/165607
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