Objectives: To investigate the presence of immunoglobulin-like transcript (ILT)4 and costimulatory proteins (CD40, CD80 and CD86), as well as tumour necrosis factor (TNF)-α production in antigen-presenting cells (APCs) from patients with psoriatic arthritis, before and after treatment with the antitumour necrosis factor-α therapy, adalimumab. Methods: Peripheral blood monocytes from patients with psoriatic arthritis and healthy controls were cultured with CD40 ligand (CD40L) to stimulate differentiation to APCs. Cell-surface phenotype was analysed via fluorescence-activated cell sorting. Results: CD40L-stimulation resulted in significantly more ILT4+ monocytes in cultures from control subjects (n = 21) than those from patients (n = 20). ILT4-positivity on CD40L-stimulated monocytes was negatively correlated with disease activity in patients. Adalimumab treatment resulted in significant increases from baseline in ILT4-positivity, and in decreases in CD40, CD80 and CD86-positivity in monocytes from patients. Conclusion: The effect of adalimumab on monocyte surface phenotype may be due to modification of the inflammatory milieu associated with therapy-induced reduction of disease activity in psoriatic arthritis.

Chimenti, M.S., Bergamini, A., Triggianese, P., Guarino, M.d., Gigliucci, G., Conigliaro, P., et al. (2016). Expression of immunoglobulin-like transcript 4 as an inhibitory receptor in patients with psoriatic arthritis. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 44(1_suppl), 22-27 [10.1177/0300060515598899].

Expression of immunoglobulin-like transcript 4 as an inhibitory receptor in patients with psoriatic arthritis

CHIMENTI, MARIA SOLE;BERGAMINI, ALBERTO;TRIGGIANESE, PAOLA;GUARINO, MARIA DOMENICA;CONIGLIARO, PAOLA;PERRICONE, ROBERTO
2016

Abstract

Objectives: To investigate the presence of immunoglobulin-like transcript (ILT)4 and costimulatory proteins (CD40, CD80 and CD86), as well as tumour necrosis factor (TNF)-α production in antigen-presenting cells (APCs) from patients with psoriatic arthritis, before and after treatment with the antitumour necrosis factor-α therapy, adalimumab. Methods: Peripheral blood monocytes from patients with psoriatic arthritis and healthy controls were cultured with CD40 ligand (CD40L) to stimulate differentiation to APCs. Cell-surface phenotype was analysed via fluorescence-activated cell sorting. Results: CD40L-stimulation resulted in significantly more ILT4+ monocytes in cultures from control subjects (n = 21) than those from patients (n = 20). ILT4-positivity on CD40L-stimulated monocytes was negatively correlated with disease activity in patients. Adalimumab treatment resulted in significant increases from baseline in ILT4-positivity, and in decreases in CD40, CD80 and CD86-positivity in monocytes from patients. Conclusion: The effect of adalimumab on monocyte surface phenotype may be due to modification of the inflammatory milieu associated with therapy-induced reduction of disease activity in psoriatic arthritis.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/16 - Reumatologia
English
Antitumour necrosis factor-α; ILT4; immunoglobulin-like transcript 4; innate immunity; psoriatic arthritis; TNF-α; Medicine (all); Biochemistry; Cell Biology; Biochemistry (medical)
http://imr.sagepub.com/content/by/year
Chimenti, M.S., Bergamini, A., Triggianese, P., Guarino, M.d., Gigliucci, G., Conigliaro, P., et al. (2016). Expression of immunoglobulin-like transcript 4 as an inhibitory receptor in patients with psoriatic arthritis. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 44(1_suppl), 22-27 [10.1177/0300060515598899].
Chimenti, Ms; Bergamini, A; Triggianese, P; Guarino, Md; Gigliucci, G; Conigliaro, P; Perricone, C; Perricone, R
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/165605
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