It has been reported that sorbitol induces apoptosis in several cancer cell lines. However, the molecular mechanism underlying the sorbitol-induced apoptotic process is not yet clearly understood. In the present study, the intracellular signaling pathways of sorbitol-induced apoptosis in human K562 cells were investigated using both morphological analysis and DNA fragmentation technique. In this study, we demonstrated that sorbitol-induced apoptosis in human K562 cells is a concentration- and time-dependent manner. This sorbitol-induced apoptosis in human K562 cells was also accompanied by the up-regulation of Bax, and down-regulation of p-Bcl-2, but no effect on the levels of Bcl-X(L). Moreover, the sorbitol treatment resulted in a significant reduction of mitochondria membrane potential, increase in the release of mitochondrial cytochrome c (cyt c), and activation of caspase 3. Furthermore, treatment with caspase 3 inhibitor (z-DEVD-fmk) was capable of preventing the sorbitol-induced caspase 3 activity and cell death. These results clearly demonstrate that the induction of apoptosis by sorbitol involves multiple cellular/molecular pathways and strongly suggest that pro- and anti-apoptotic Bcl-2 family proteins, mitochondrial membrane potential, mitochondrial cyt c, and caspase 3, they all participate in sorbitol-induced apoptotic process in human K562 cells.

Marfè, G., Morgante, E., DI STEFANO, C., DI RENZO, L., DE MARTINO, L., Iovane, G., et al. (2008). Sorbitol-induced apoptosis of human leukemia is mediated by caspase activation and cytochrome c release. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol. 82(6), 371-377.

Sorbitol-induced apoptosis of human leukemia is mediated by caspase activation and cytochrome c release.

SINIBALDI SALIMEI, PAOLA
2008-01-01

Abstract

It has been reported that sorbitol induces apoptosis in several cancer cell lines. However, the molecular mechanism underlying the sorbitol-induced apoptotic process is not yet clearly understood. In the present study, the intracellular signaling pathways of sorbitol-induced apoptosis in human K562 cells were investigated using both morphological analysis and DNA fragmentation technique. In this study, we demonstrated that sorbitol-induced apoptosis in human K562 cells is a concentration- and time-dependent manner. This sorbitol-induced apoptosis in human K562 cells was also accompanied by the up-regulation of Bax, and down-regulation of p-Bcl-2, but no effect on the levels of Bcl-X(L). Moreover, the sorbitol treatment resulted in a significant reduction of mitochondria membrane potential, increase in the release of mitochondrial cytochrome c (cyt c), and activation of caspase 3. Furthermore, treatment with caspase 3 inhibitor (z-DEVD-fmk) was capable of preventing the sorbitol-induced caspase 3 activity and cell death. These results clearly demonstrate that the induction of apoptosis by sorbitol involves multiple cellular/molecular pathways and strongly suggest that pro- and anti-apoptotic Bcl-2 family proteins, mitochondrial membrane potential, mitochondrial cyt c, and caspase 3, they all participate in sorbitol-induced apoptotic process in human K562 cells.
2008
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/05 - PATOLOGIA CLINICA
English
Con Impact Factor ISI
Marfè, G., Morgante, E., DI STEFANO, C., DI RENZO, L., DE MARTINO, L., Iovane, G., et al. (2008). Sorbitol-induced apoptosis of human leukemia is mediated by caspase activation and cytochrome c release. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol. 82(6), 371-377.
Marfè, G; Morgante, E; DI STEFANO, C; DI RENZO, L; DE MARTINO, L; Iovane, G; Russo, M; SINIBALDI SALIMEI, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/16355
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