Multicentric Castleman's disease (MCD) and primary effusion lymphoma (PEL) are two B-cell lymphoproliferative diseases associated with Kaposi's sarcoma-associated herpes virus/human herpesvirus-8 (KSHV/HHV-8). Although MCD is considered a prelymphoma state, it is not known whether a pathogenetic link exists between MCD and PEL. This paper reports the clinico-pathological features of four cases of PEL (two pericardial, one pleural, and one peritoneal) developing in the context of HIV-associated MCD. Effusions, lymph nodes, spleen, and additional tissues from three autopsies were examined for morphology/immunophenotype, search for HHV-8 DNA, and assessment of immunoglobulin heavy chain gene (IgH) configuration using polymerase chain reaction (PCR)-based techniques. MCD and PEL samples contained HHV-8 DNA. Clonal IgH rearrangements were detected only in PEL, whereas MCD tissues were polyclonal. Light-chain immunostaining confirmed B-cell clonality in PEL (two lambda, one kappa, one not tested) and polyclonality in MCD. The autopsies revealed different morphological variants of visceral KS and multi-organ atypical infiltrates exhibiting immunoblastic/plasmablastic features reminiscent of PEL morphology, with a restriction of lambda-positive cells. In two cases, using microdissection and IgH PCR analysis, multiple/discrete bands were found in the infiltrates, compatible with polyclonality/oligoclonality. The case showing an oligoclonal IgH ladder contained a rearrangement of identical junctional size to the PEL clone; however, further analysis with PEL-derived clonotypic primers and sequencing of PCR products showed no amplification and nucleotide diversity, respectively, indicating that the two B-cell populations examined were clonally unrelated. These data show that MCD and PEL may co-exist in HIV-infected patients, suggesting a relevant association between these two HHV-8-related disorders. Although a definite clonal relationship between MCD and PEL was not demonstrated, it is hypothesized that in some MCD cases, within expanded polyclonal B-cell populations secondary to HHV-8 infection, clonal expansions may occur that localize into a body cavity, i.e. PEL.
Ascoli, V., Signoretti, S., Onetti-Muda, A., Pescarmona, E., Della-Rocca, C., Nardi, F., et al. (2001). Primary effusion lymphoma in HIV-infected patients with multicentric Castleman's disease. JOURNAL OF PATHOLOGY, 193(2), 200-9-209.
|Tipologia:||Articolo su rivista|
|Citazione:||Ascoli, V., Signoretti, S., Onetti-Muda, A., Pescarmona, E., Della-Rocca, C., Nardi, F., et al. (2001). Primary effusion lymphoma in HIV-infected patients with multicentric Castleman's disease. JOURNAL OF PATHOLOGY, 193(2), 200-9-209.|
|Settore Scientifico Disciplinare:||Settore MED/15 - Malattie del Sangue|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/1096-9896(200102)193:2<200::AID-PATH773>3.0.CO;2-L|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||feb-2001|
|Titolo:||Primary effusion lymphoma in HIV-infected patients with multicentric Castleman's disease|
|Autori:||Ascoli, V; Signoretti, S; Onetti-Muda, A; Pescarmona, E; Della-Rocca, C; Nardi, F; Mastroianni, Cm; Gastaldi, R; Pistilli, A; Gaidano, G; Carbone, A; Lo Coco, F|
|Appare nelle tipologie:||01 - Articolo su rivista|