The detection of PML-RAR by reverse transcription (RT) polymerase chain reaction (PCR) in acute promyelocytic leukemia (APL) patients who are in hematologic remission influences therapeutic decision making in several trials. In the light of this, the Spanish group has recently designed an external quality assessment program (EQAP) of RT-PCR detection of PML-RAR, which includes a study of sensitivity of the participating laboratories.

Bolufer, P., LO COCO, F., Grimwade, D., Barragán, E., Diverio, D., Cassinat, B., et al. (2001). Variability in the levels of PML-RAR alpha fusion transcripts detected by the laboratories participating in an external quality control program using several reverse transcription polymerase chain reaction protocols. HAEMATOLOGICA, 86(6), 570-6.

Variability in the levels of PML-RAR alpha fusion transcripts detected by the laboratories participating in an external quality control program using several reverse transcription polymerase chain reaction protocols

LO COCO, FRANCESCO;
2001-06-01

Abstract

The detection of PML-RAR by reverse transcription (RT) polymerase chain reaction (PCR) in acute promyelocytic leukemia (APL) patients who are in hematologic remission influences therapeutic decision making in several trials. In the light of this, the Spanish group has recently designed an external quality assessment program (EQAP) of RT-PCR detection of PML-RAR, which includes a study of sensitivity of the participating laboratories.
giu-2001
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/15 - MALATTIE DEL SANGUE
English
Humans; Laboratories; Neoplasm Proteins; Observer Variation; Oncogene Proteins, Fusion; Quality Control; RNA, Messenger; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured
Bolufer, P., LO COCO, F., Grimwade, D., Barragán, E., Diverio, D., Cassinat, B., et al. (2001). Variability in the levels of PML-RAR alpha fusion transcripts detected by the laboratories participating in an external quality control program using several reverse transcription polymerase chain reaction protocols. HAEMATOLOGICA, 86(6), 570-6.
Bolufer, P; LO COCO, F; Grimwade, D; Barragán, E; Diverio, D; Cassinat, B; Chomienne, C; Gonzalez, M; Colomer, D; Gomez, M; Marugan, I; Román, J; Delgado, M; García Marco, J; Bornstein, R; Vizmanos, J; Martinez, B; Jansen, J; Villegas, A; de Blas, J; Cabello, P; Sanz, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/161943
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