In this study we used reverse transcriptase-polymerase chain reaction (RT-PCR) for the longitudinal monitoring of minimal residual disease in 12 patients with All-1/AF-4 positive ALL. Of these, seven also showed at presentation a typical t(4;11) cytogenetic translocation. Seven patients were infants <18 months of age and five were adults. Eleven patients were treated with high-dose intensive induction and consolidation chemotherapy without bone marrow transplantation and one received conservative treatment due to poor performance status. Three had resistant disease, four relapsed within 12 months after achieving complete remission, and five are in continuous complete remission (CCR) at 32, 39, 52, 53 and 61 months from diagnosis, respectively. The sequential analysis of the ALL-1/AF-4 hybrid transcript showed a persistently negative RT-PCR in the five CCR long-term survivors. The PCR analysis resulted persistently positive in the remaining seven cases, including the four cases who relapsed after the achievement of clinical CR. These data emphasize the clinical relevance of PCR monitoring analysis in t(4;11) ALL patients and should be considered in order to better determine variable post-remission treatment according to risk prediction.

Cimino, G., Elia, L., Rivolta, A., Rapanotti, M.c., Rossi, V., Alimena, G., et al. (1996). Clinical relevance of residual disease monitoring by polymerase chain reaction in patients with ALL-1/AF-4 positive-acute lymphoblastic leukaemia. BRITISH JOURNAL OF HAEMATOLOGY, 92(3), 659-664.

Clinical relevance of residual disease monitoring by polymerase chain reaction in patients with ALL-1/AF-4 positive-acute lymphoblastic leukaemia

LO COCO, FRANCESCO;
1996-03

Abstract

In this study we used reverse transcriptase-polymerase chain reaction (RT-PCR) for the longitudinal monitoring of minimal residual disease in 12 patients with All-1/AF-4 positive ALL. Of these, seven also showed at presentation a typical t(4;11) cytogenetic translocation. Seven patients were infants <18 months of age and five were adults. Eleven patients were treated with high-dose intensive induction and consolidation chemotherapy without bone marrow transplantation and one received conservative treatment due to poor performance status. Three had resistant disease, four relapsed within 12 months after achieving complete remission, and five are in continuous complete remission (CCR) at 32, 39, 52, 53 and 61 months from diagnosis, respectively. The sequential analysis of the ALL-1/AF-4 hybrid transcript showed a persistently negative RT-PCR in the five CCR long-term survivors. The PCR analysis resulted persistently positive in the remaining seven cases, including the four cases who relapsed after the achievement of clinical CR. These data emphasize the clinical relevance of PCR monitoring analysis in t(4;11) ALL patients and should be considered in order to better determine variable post-remission treatment according to risk prediction.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/15 - Malattie del Sangue
English
Adult; Antineoplastic Agents; Base Sequence; Chromosomes, Human, Pair 11; Chromosomes, Human, Pair 4; DNA-Binding Proteins; Female; Histone-Lysine N-Methyltransferase; Humans; Infant; Longitudinal Studies; Male; Middle Aged; Molecular Sequence Data; Myeloid-Lymphoid Leukemia Protein; Neoplasm, Residual; Nuclear Proteins; Polymerase Chain Reaction; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Prognosis; RNA-Directed DNA Polymerase; Recombinant Fusion Proteins; Retrospective Studies; Translocation, Genetic; Proto-Oncogenes; Transcription Factors
Cimino, G., Elia, L., Rivolta, A., Rapanotti, M.c., Rossi, V., Alimena, G., et al. (1996). Clinical relevance of residual disease monitoring by polymerase chain reaction in patients with ALL-1/AF-4 positive-acute lymphoblastic leukaemia. BRITISH JOURNAL OF HAEMATOLOGY, 92(3), 659-664.
Cimino, G; Elia, L; Rivolta, A; Rapanotti, M; Rossi, V; Alimena, G; Annino, L; Canaani, E; LO COCO, F; Biondi, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/161712
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