Repeated PCR analysis was performed on bone marrow and/or peripheral blood samples from 4 CML patients in complete cytogenetic remission during treatment with IFN-alpha. Two patients became PCR-negative. One was negative for the analyses carried out from the 9th to the 30th months, but reverted to PCR positivity 10 months after IFN was reduced from 1.5 x 10(6) IU/day to 1 x 10(6) IU and given on alternate days. Although the dose was again raised to 3 x 10(6) IU/day, 8 months later her peripheral blood cells were still PCR-positive, but remained persistently Ph'-negative. Another patient became PCR-negative at the 42nd month and remained so at the last analysis performed 3 months later. Two patients were persistently PCR-positive. Cytogenetic relapse was documented in both, in one while still on full therapy. Ph'-positive metaphases reappeared in the other patient 7 months after discontinuing IFN-alpha therapy.

Liberati, A., Donti, E., Rosso, C., LO COCO, F., Dore, F., Pardini, S., et al. (1994). Repeated PCR in CML during IFN-alpha therapy. EUROPEAN JOURNAL OF HAEMATOLOGY, 52(3), 152-155.

Repeated PCR in CML during IFN-alpha therapy

LO COCO, FRANCESCO;
1994-03-01

Abstract

Repeated PCR analysis was performed on bone marrow and/or peripheral blood samples from 4 CML patients in complete cytogenetic remission during treatment with IFN-alpha. Two patients became PCR-negative. One was negative for the analyses carried out from the 9th to the 30th months, but reverted to PCR positivity 10 months after IFN was reduced from 1.5 x 10(6) IU/day to 1 x 10(6) IU and given on alternate days. Although the dose was again raised to 3 x 10(6) IU/day, 8 months later her peripheral blood cells were still PCR-positive, but remained persistently Ph'-negative. Another patient became PCR-negative at the 42nd month and remained so at the last analysis performed 3 months later. Two patients were persistently PCR-positive. Cytogenetic relapse was documented in both, in one while still on full therapy. Ph'-positive metaphases reappeared in the other patient 7 months after discontinuing IFN-alpha therapy.
mar-1994
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/15 - MALATTIE DEL SANGUE
English
Adult; Base Sequence; Bone Marrow; DNA Primers; DNA, Neoplasm; Female; Fusion Proteins, bcr-abl; Humans; Interferon-alpha; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukocytes; Male; Molecular Sequence Data; Polymerase Chain Reaction
Liberati, A., Donti, E., Rosso, C., LO COCO, F., Dore, F., Pardini, S., et al. (1994). Repeated PCR in CML during IFN-alpha therapy. EUROPEAN JOURNAL OF HAEMATOLOGY, 52(3), 152-155.
Liberati, A; Donti, E; Rosso, C; LO COCO, F; Dore, F; Pardini, S; Cocco, S; Addis, M; Saglio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/161552
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