Unlike in Escherichia coli, in Salmonella enterica production of class B acid phosphatase (AphA) was detectable also in cells growing in the presence of glucose. Characterization of the aphA locus from a S. enterica ser. typhi strain showed that the aphA determinant is very similar to the E. coli homolog, and that its chromosomal location between the highly conserved tyrB and uvrA genes is retained. However, the aphA flanking regions were found to be markedly different in the two species, either between tyrB and aphA or between aphA and uvrA. The differences in the aphA 5'-flanking region, which in S. enterica is considerably shorter than in E. coli (183 vs. 1121 bp) and includes potential promoter sequences not present in E. coli, could be responsible for the different regulation of class B acid phosphatase observed in the two species.

Thaller, M.C., Schippa, S., Bonci, A., Berlutti, F., Selan, L., & Rossolini, G.M. (1999). Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli. FEMS MICROBIOLOGY LETTERS, 181(1), 17-23.

Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli

THALLER, MARIA CRISTINA;
1999-12-01

Abstract

Unlike in Escherichia coli, in Salmonella enterica production of class B acid phosphatase (AphA) was detectable also in cells growing in the presence of glucose. Characterization of the aphA locus from a S. enterica ser. typhi strain showed that the aphA determinant is very similar to the E. coli homolog, and that its chromosomal location between the highly conserved tyrB and uvrA genes is retained. However, the aphA flanking regions were found to be markedly different in the two species, either between tyrB and aphA or between aphA and uvrA. The differences in the aphA 5'-flanking region, which in S. enterica is considerably shorter than in E. coli (183 vs. 1121 bp) and includes potential promoter sequences not present in E. coli, could be responsible for the different regulation of class B acid phosphatase observed in the two species.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/19 - Microbiologia Generale
English
Acid Phosphatase; Adenosine Triphosphatases; Bacterial Proteins; Base Sequence; Cosmids; DNA-Binding Proteins; Escherichia coli; Genes, Bacterial; Glucose; Molecular Sequence Data; Restriction Mapping; Salmonella enterica; Sequence Alignment; Sequence Analysis, DNA; Chromosome Mapping; Escherichia coli Proteins; Gene Expression Regulation, Bacterial
Thaller, M.C., Schippa, S., Bonci, A., Berlutti, F., Selan, L., & Rossolini, G.M. (1999). Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli. FEMS MICROBIOLOGY LETTERS, 181(1), 17-23.
Thaller, Mc; Schippa, S; Bonci, A; Berlutti, F; Selan, L; Rossolini, G
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
1999 nap sty.pdf

accesso aperto

Descrizione: Free Article
Licenza: Copyright dell'editore
Dimensione 263.66 kB
Formato Adobe PDF
263.66 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/156587
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact