In this study, we report evidences that Mycobacterium tuberculosis (MTB)-induced apoptosis in macrophages is reduced by a broad-spectrum hydroxamic acid-based matrix metalloproteinase (MMP) inhibitor, Batimastat (BB-94). In particular, we show that BB-94 administration to MTB-infected macrophages inhibits apoptosis and the downmodulation of membrane CD14 expression. Moreover, the addition of broad spectrum matrix metalloproteinase inhibitor to cell culture, during MTB infection, decreases the release of soluble TNF-alpha and leads to a simultaneous increase of membrane TNF-alpha. These results show that MTB-induced apoptosis in macrophages is reduced by a MMP inhibitor and most probably is related to TNF-alpha release. This identifies BB-94 as a simultaneous anti-apoptotic and anti-inflammatory molecule during MTB infection.

Santucci, M.b., Ciaramella, A., Mattei, M., Sumerska, T., Fraziano, M. (2003). Batimastat reduces Mycobacterium tuberculosis-induced apoptosis in macrophages. INTERNATIONAL IMMUNOPHARMACOLOGY, 3(12), 1657-1665 [10.1016/S1567-5769(03)00202-9].

Batimastat reduces Mycobacterium tuberculosis-induced apoptosis in macrophages

SANTUCCI, MARILINA BENEDETTA;MATTEI, MAURIZIO;FRAZIANO, MAURIZIO
2003-11-01

Abstract

In this study, we report evidences that Mycobacterium tuberculosis (MTB)-induced apoptosis in macrophages is reduced by a broad-spectrum hydroxamic acid-based matrix metalloproteinase (MMP) inhibitor, Batimastat (BB-94). In particular, we show that BB-94 administration to MTB-infected macrophages inhibits apoptosis and the downmodulation of membrane CD14 expression. Moreover, the addition of broad spectrum matrix metalloproteinase inhibitor to cell culture, during MTB infection, decreases the release of soluble TNF-alpha and leads to a simultaneous increase of membrane TNF-alpha. These results show that MTB-induced apoptosis in macrophages is reduced by a MMP inhibitor and most probably is related to TNF-alpha release. This identifies BB-94 as a simultaneous anti-apoptotic and anti-inflammatory molecule during MTB infection.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - Patologia Generale
English
Con Impact Factor ISI
HLA-DR Antigens; Phenylalanine; Antigens, CD14; Cells, Cultured; Leukocytes, Mononuclear; Thiophenes; Humans; Flow Cytometry; Tumor Necrosis Factor-alpha; Apoptosis; Annexin A5; Macrophages; Propidium; Mycobacterium tuberculosis; Interleukin-6
Santucci, M.b., Ciaramella, A., Mattei, M., Sumerska, T., Fraziano, M. (2003). Batimastat reduces Mycobacterium tuberculosis-induced apoptosis in macrophages. INTERNATIONAL IMMUNOPHARMACOLOGY, 3(12), 1657-1665 [10.1016/S1567-5769(03)00202-9].
Santucci, Mb; Ciaramella, A; Mattei, M; Sumerska, T; Fraziano, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/15456
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