Neuronal redox phenomena are involved in numerous biochemical pathways and play a key role in many pathological events and clinical situations. The oxidation/reduction (redox) state present in biological compartments is a major target for possible pharmaceutical intervention and, consequently, the processes associated with its change have attracted increased attention in recent years. Here, we analyze the redox environment and its spatial compartmentalization in differentiated neuronal phenotype of PC-12 cells using a redox-sensitive protein (i.e., a mutant of the Yellow Fluorescent protein), employed ratiometrically. Redox maps of cells were generated with an elevate spatial resolution, and the spatial distributions of highly oxidized and highly reduced regions have been determined. A quantitative analysis of redox maps allows the disclosure of a peculiar spatial organization of the redox environment.

Maulucci, G., Pani, G., Fusco, S., Papi, M., Arcovito, G., Galeotti, T., et al. (2010). Compartmentalization of the redox environment in PC-12 neuronal cells. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 39(6), 993-999 [10.1007/s00249-009-0470-9].

Compartmentalization of the redox environment in PC-12 neuronal cells

FRAZIANO, MAURIZIO;
2010-05-01

Abstract

Neuronal redox phenomena are involved in numerous biochemical pathways and play a key role in many pathological events and clinical situations. The oxidation/reduction (redox) state present in biological compartments is a major target for possible pharmaceutical intervention and, consequently, the processes associated with its change have attracted increased attention in recent years. Here, we analyze the redox environment and its spatial compartmentalization in differentiated neuronal phenotype of PC-12 cells using a redox-sensitive protein (i.e., a mutant of the Yellow Fluorescent protein), employed ratiometrically. Redox maps of cells were generated with an elevate spatial resolution, and the spatial distributions of highly oxidized and highly reduced regions have been determined. A quantitative analysis of redox maps allows the disclosure of a peculiar spatial organization of the redox environment.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - Patologia Generale
Settore BIO/10
English
Con Impact Factor ISI
Cell Line; Cell Compartmentation; Neurons; PC12 Cells; Nerve Tissue Proteins; Cells; Rats; Biosensing Techniques; Animals; Oxidation-Reduction; Microscopy, Confocal
Maulucci, G., Pani, G., Fusco, S., Papi, M., Arcovito, G., Galeotti, T., et al. (2010). Compartmentalization of the redox environment in PC-12 neuronal cells. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 39(6), 993-999 [10.1007/s00249-009-0470-9].
Maulucci, G; Pani, G; Fusco, S; Papi, M; Arcovito, G; Galeotti, T; Fraziano, M; De Spirito, M
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/15448
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