CINECA IRIS Institutional Research Information System
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishTwo-dimensional 1H-NMR spectra were carried out on bovine Cu(I),Zn superoxide dismutase. The ring protons of the single tyrosine and of the 4 phenylalanines were identified from COSY spectra. From NOESY spectra all imidazole C-resonances could be specifically assigned to each of the 8 histidines using the crystal coordinates of the Cu(II),Zn enzyme. Since 6 histidines are involved in the structure of the active site, this result implies nearly identical active site conformations for the two oxidation states of the catalytic cycle of this enzyme, in line with its diffusion-limited rate.
Paci, M., Desideri, A., Sette, M., Ciriolo, M.R., & Rotilio, G. (1990). Assignment of imidazole resonances from two-dimensional proton NMR spectra of bovine Cu,Zn superoxide dismutase. Evidence for similar active site conformation in the oxidized and reduced enzyme. FEBS LETTERS, 263(1), 127-130.
| Tipologia: | Articolo su rivista | |
| Citazione: | Paci, M., Desideri, A., Sette, M., Ciriolo, M.R., & Rotilio, G. (1990). Assignment of imidazole resonances from two-dimensional proton NMR spectra of bovine Cu,Zn superoxide dismutase. Evidence for similar active site conformation in the oxidized and reduced enzyme. FEBS LETTERS, 263(1), 127-130. | |
| URL: | http://www.mendeley.com/research/assignment-imidazole-resonances-twodimensional-proton-nmr-spectra-bovine-cuzn-superoxide-dismutase-evidence-similar-active-site-conformation-oxidized-reduced-enzyme/ | |
| IF: | Con Impact Factor ISI | |
| Lingua: | English | |
| Settore Scientifico Disciplinare: | Settore BIO/10 | |
| Revisione (peer review): | Sì, ma tipo non specificato | |
| Tipo: | Articolo | |
| Rilevanza: | Rilevanza internazionale | |
| Stato di pubblicazione: | Pubblicato | |
| Data di pubblicazione: | 9-apr-1990 | |
| Titolo: | Assignment of imidazole resonances from two-dimensional proton NMR spectra of bovine Cu,Zn superoxide dismutase. Evidence for similar active site conformation in the oxidized and reduced enzyme | |
| Autori: | ||
| Autori: | Paci, M; Desideri, A; Sette, M; Ciriolo, MR; Rotilio, G | |
| Appare nelle tipologie: | 01 - Articolo su rivista |
File in questo prodotto:
Copyright © 2021