We found that the binding of [3H]prazosin, a selective ligand for alpha 1-adrenergic recognition sites, is significantly lower in the frontal cortex of the genetically epilepsy-prone rats (GEPRs), as compared with normal Sprague-Dawley rats. Scatchard analysis reveals a decrease in the Bmax of [3H]prazosin binding with no change in the apparent KD, suggesting that there are fewer alpha 1-adrenergic recognition sites in the frontal cortex of the GEPR. This abnormality is associated with a reduced capacity of norepinephrine (NE) to stimulate [3H]inositol monophosphate ([3H]IP1) formation in frontal cortex slices prelabeled with [3H]inositol. No significant differences in [3H]prazosin binding as well as NE-stimulated [3H]IP1 formation have been observed in other brain regions including hippocampus, corpus striatum, and inferior colliculus. These results indicate that a deficit in the alpha 1-adrenergic receptor system in the frontal cortex may play a role in the seizure process in the GEPR.

Nicoletti, F., Barbaccia, M.l., Iadarola, M., Pozzi, O., Laird, H. (1986). Abnormality of alpha 1-adrenergic receptors in the frontal cortex of epileptic rats. JOURNAL OF NEUROCHEMISTRY, 46(1), 270-273.

Abnormality of alpha 1-adrenergic receptors in the frontal cortex of epileptic rats

BARBACCIA, MARIA LUISA;
1986-01-01

Abstract

We found that the binding of [3H]prazosin, a selective ligand for alpha 1-adrenergic recognition sites, is significantly lower in the frontal cortex of the genetically epilepsy-prone rats (GEPRs), as compared with normal Sprague-Dawley rats. Scatchard analysis reveals a decrease in the Bmax of [3H]prazosin binding with no change in the apparent KD, suggesting that there are fewer alpha 1-adrenergic recognition sites in the frontal cortex of the GEPR. This abnormality is associated with a reduced capacity of norepinephrine (NE) to stimulate [3H]inositol monophosphate ([3H]IP1) formation in frontal cortex slices prelabeled with [3H]inositol. No significant differences in [3H]prazosin binding as well as NE-stimulated [3H]IP1 formation have been observed in other brain regions including hippocampus, corpus striatum, and inferior colliculus. These results indicate that a deficit in the alpha 1-adrenergic receptor system in the frontal cortex may play a role in the seizure process in the GEPR.
gen-1986
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14 - FARMACOLOGIA
English
Con Impact Factor ISI
Receptors, Adrenergic, alpha; Hippocampus; Male; Synaptic Membranes; Inferior Colliculi; Rats; Dose-Response Relationship, Drug; Norepinephrine; Phosphatidylinositols; Yohimbine; Animals; Cerebral Cortex; Propranolol; Phentolamine; Carbachol; Rats, Mutant Strains; Rats, Inbred Strains; Clonidine; Epilepsy; Prazosin
Nicoletti, F., Barbaccia, M.l., Iadarola, M., Pozzi, O., Laird, H. (1986). Abnormality of alpha 1-adrenergic receptors in the frontal cortex of epileptic rats. JOURNAL OF NEUROCHEMISTRY, 46(1), 270-273.
Nicoletti, F; Barbaccia, Ml; Iadarola, M; Pozzi, O; Laird, H
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/14991
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