Caspase-14 is a recent addition to the caspase family of aspartate proteases involved in apoptotic processes. Human caspase-14 appears to be only weakly processed during apoptosis, and it does not cleave classical caspase substrates. Post partum, caspase-14 is prominently expressed by human keratinocytes and reportedly participates in terminal differentiation of complex epithelia. Here we provide evidence challenging the view that caspase-14 expression or processing is linked exclusively to terminal keratinocyte differentiation. We demonstrate that caspase-14 expression extended to multiple cell lines derived from simple epithelia of the breast, prostate, and stomach. In keratinocytes and breast epithelial cells, caspase-14 expression was upregulated in high-density cultures and during forced suspension culture. These effects were primarily due to transcriptional activation as indicated by reporter gene assays using a 2 kb caspase-14 promoter fragment. Importantly, caspase-14 was not cleaved during forced suspension culture of either cell type although this treatment induced caspase-dependent apoptosis (anoikis). Forced expression of caspase-14 in immortalized human keratinocytes had no effect on cell death in forced suspension nor was the transfected caspase-14 processed in this setting. In contrast to postconfluent and forced suspension culture, terminal differentiation of keratinocytes induced in vitro by Ca2+ treatment was not associated with increased caspase-14 expression or promoter activity. Our results indicate that (1) caspase-14 is expressed not only in complex but also simple epithelia; (2) cells derived from complex and simple epithelia upregulate caspase-14 expression in conditions of high cell density or lack of matrix interaction and; (3) in both cell types this phenomenon is due to transcriptional regulation.
Pistritto, G., Jost, M., Srinivasula, S.M., Baffa, R., Poyet, J., Kari, C., et al. (2002). Expression and transcriptional regulation of caspase-14 in simple and complex epithelia. CELL DEATH AND DIFFERENTIATION, 9(9), 995-1006.
|Tipologia:||Articolo su rivista|
|Citazione:||Pistritto, G., Jost, M., Srinivasula, S.M., Baffa, R., Poyet, J., Kari, C., et al. (2002). Expression and transcriptional regulation of caspase-14 in simple and complex epithelia. CELL DEATH AND DIFFERENTIATION, 9(9), 995-1006.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/14|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/sj.cdd.4401061|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||set-2002|
|Titolo:||Expression and transcriptional regulation of caspase-14 in simple and complex epithelia|
|Autori:||Pistritto, G; Jost, M; Srinivasula, SM; Baffa, R; Poyet, J; Kari, C; Lazebnik, Y; Rodeck, U; Alnemri, ES|
|Appare nelle tipologie:||01 - Articolo su rivista|