In the present research protein analysis on SDS-polyacrylamide gel electrophoresis (PAGE) has been used to study the glycoprotein pattern of the blood platelets of four members from a family affected by May-Hegglin anomaly. In order to characterize the glycoprotein fractions, lactoperoxidase-iodination and immunoprecipitation procedures were used. N,N'diallyltartardiamide (DATD) cross-linked gel electrophoresis was shown to improve the glycoprotein pattern resolution, although with the lactoperoxidase-iodination the glycoprotein characterization of May-Hegglin platelets overlapped the normal. On the other hand with the immunoprecipitation the specific antiserum precipitated all the five major fractions of normal membrane glycoproteins, but it was shown to react quite poorly with the component V of the May-Hegglin glycoprotein pattern.

Ricci, G., Manservigi, R., ALBONICI BOVE, L., Zavagli, G., Cassai, E. (1985). Evidence for glycoprotein abnormality in platelets from patients with May-Hegglin anomaly. THROMBOSIS AND HAEMOSTASIS, 54(4), 862-865.

Evidence for glycoprotein abnormality in platelets from patients with May-Hegglin anomaly

ALBONICI BOVE, LOREDANA;
1985-12-17

Abstract

In the present research protein analysis on SDS-polyacrylamide gel electrophoresis (PAGE) has been used to study the glycoprotein pattern of the blood platelets of four members from a family affected by May-Hegglin anomaly. In order to characterize the glycoprotein fractions, lactoperoxidase-iodination and immunoprecipitation procedures were used. N,N'diallyltartardiamide (DATD) cross-linked gel electrophoresis was shown to improve the glycoprotein pattern resolution, although with the lactoperoxidase-iodination the glycoprotein characterization of May-Hegglin platelets overlapped the normal. On the other hand with the immunoprecipitation the specific antiserum precipitated all the five major fractions of normal membrane glycoproteins, but it was shown to react quite poorly with the component V of the May-Hegglin glycoprotein pattern.
17-dic-1985
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/46 - SCIENZE TECNICHE DI MEDICINA E DI LABORATORIO
English
blood platelets; female; membrane proteins; electrophoresis, polyacrylamide gel; antigens; glycoproteins; leukocytes; humans; hematologic diseases
Ricci, G., Manservigi, R., ALBONICI BOVE, L., Zavagli, G., Cassai, E. (1985). Evidence for glycoprotein abnormality in platelets from patients with May-Hegglin anomaly. THROMBOSIS AND HAEMOSTASIS, 54(4), 862-865.
Ricci, G; Manservigi, R; ALBONICI BOVE, L; Zavagli, G; Cassai, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/14983
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