Two insulin receptor mRNA species are expressed in human tissues as a result of alternative splicing of exon 11. This event is regulated in a tissue-specific manner. To date, there is little information about the relative abundance of the two receptor protein isoforms on the cell surface. The aim of the present investigation was to assess whether the tissue-specific expression of the two insulin receptor mRNA species is paralleled by a similar pattern of expression of the two receptor protein isoforms. To this end, we assessed the relative distribution of the two receptor variants in various human tissues at the mRNA and protein levels. A PCR-based technique was used to measure the relative abundance of the two mRNA species, and two immunological assays were used to measure the relative steady-state expression of the two receptor protein isoforms. The expression of the two insulin receptor protein isoforms followed the tissue-specific pattern of expression of the two mRNA species.

Sesti, G., Tullio, A., D'Alfonso, R., Napolitano, M., Marini, M.a., Borboni, P., et al. (1994). Tissue-specific expression of two alternatively spliced isoforms of the human insulin receptor protein. ACTA DIABETOLOGICA, 31(2), 59-65.

Tissue-specific expression of two alternatively spliced isoforms of the human insulin receptor protein

D'ALFONSO, ROSSELLA;MARINI, MARIA ADELAIDE;BORBONI, PATRIZIA;ALBONICI BOVE, LOREDANA;FUSCO, ANGELO;
1994-06-01

Abstract

Two insulin receptor mRNA species are expressed in human tissues as a result of alternative splicing of exon 11. This event is regulated in a tissue-specific manner. To date, there is little information about the relative abundance of the two receptor protein isoforms on the cell surface. The aim of the present investigation was to assess whether the tissue-specific expression of the two insulin receptor mRNA species is paralleled by a similar pattern of expression of the two receptor protein isoforms. To this end, we assessed the relative distribution of the two receptor variants in various human tissues at the mRNA and protein levels. A PCR-based technique was used to measure the relative abundance of the two mRNA species, and two immunological assays were used to measure the relative steady-state expression of the two receptor protein isoforms. The expression of the two insulin receptor protein isoforms followed the tissue-specific pattern of expression of the two mRNA species.
giu-1994
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - Patologia Generale
Settore BIO/12
English
3T3 Cells; Placenta; Alternative Splicing; Gene Expression; Autoantibodies; Tumor Cells, Cultured; Receptor, Insulin; Mice; Pregnancy; DNA Primers; Adipose Tissue; Molecular Sequence Data; Carcinoma, Squamous Cell; Organ Specificity; Cell Line; Hypoglycemia; Female; Base Sequence; Animals; Lupus Erythematosus, Systemic; Humans; Carcinoma, Hepatocellular; Liver; RNA, Messenger; Polymerase Chain Reaction; Lymphocytes
Sesti, G., Tullio, A., D'Alfonso, R., Napolitano, M., Marini, M.a., Borboni, P., et al. (1994). Tissue-specific expression of two alternatively spliced isoforms of the human insulin receptor protein. ACTA DIABETOLOGICA, 31(2), 59-65.
Sesti, G; Tullio, A; D'Alfonso, R; Napolitano, M; Marini, Ma; Borboni, P; Longhi, R; ALBONICI BOVE, L; Fusco, A; Aglianò, A
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
1994 Sesti acta diab.pdf

accesso solo dalla rete interna

Licenza: Copyright dell'editore
Dimensione 3.02 MB
Formato Adobe PDF
3.02 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/14902
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 23
  • ???jsp.display-item.citation.isi??? ND
social impact