The possibility that dehydroepiandrosterone (DHEA) is metabolized in human plasma was studied by column and thin-layer chromatography. The results obtained indicate that a time-dependent disappearance of DHEA is matched by the appearance of newly-formed species that may represent DHEA conversion by-products. Neither disappearance of DHEA, nor formation of the alleged conversion by-products was observed when reactions were performed under conditions in which plasma enzymes were removed or inactivated. These results suggest that, in plasma, DHEA is partially transformed into different substances, and that the conversion reactions are catalyzed by enzymes present in this tissue. The observed kinetics of appearance and partial disappearance of the radiolabeled species can be interpreted as indicating that some of the by-products formed are further converted into other substances. The data shown appear to indicate that plasma can be added to the list of the already known compartments that are involved in steroid metabolism.

Albo, F., Barbaccia, M.L., Cavazza, A., Marini, M., & Roda, L.G. (2003). Dehydroepiandrosterone metabolism in human plasma. HORMONE AND METABOLIC RESEARCH, 35(2), 104-108 [10.1055/s-2003-39052].

Dehydroepiandrosterone metabolism in human plasma

BARBACCIA, MARIA LUISA;CAVAZZA, ANTONELLA;MARINI, MARIO;RODA, LUIGI GIORGIO
2003-02

Abstract

The possibility that dehydroepiandrosterone (DHEA) is metabolized in human plasma was studied by column and thin-layer chromatography. The results obtained indicate that a time-dependent disappearance of DHEA is matched by the appearance of newly-formed species that may represent DHEA conversion by-products. Neither disappearance of DHEA, nor formation of the alleged conversion by-products was observed when reactions were performed under conditions in which plasma enzymes were removed or inactivated. These results suggest that, in plasma, DHEA is partially transformed into different substances, and that the conversion reactions are catalyzed by enzymes present in this tissue. The observed kinetics of appearance and partial disappearance of the radiolabeled species can be interpreted as indicating that some of the by-products formed are further converted into other substances. The data shown appear to indicate that plasma can be added to the list of the already known compartments that are involved in steroid metabolism.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14
eng
Con Impact Factor ISI
Kinetics; Middle Aged; Tritium; Biotransformation; Chromatography, Thin Layer; Adult; Reference Values; Radioisotope Dilution Technique; Humans; Dehydroepiandrosterone
Albo, F., Barbaccia, M.L., Cavazza, A., Marini, M., & Roda, L.G. (2003). Dehydroepiandrosterone metabolism in human plasma. HORMONE AND METABOLIC RESEARCH, 35(2), 104-108 [10.1055/s-2003-39052].
Albo, F; Barbaccia, Ml; Cavazza, A; Marini, M; Roda, Lg
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/14898
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