Amyloid-beta 1-42 (Abeta1-42), peptide detectable in cerebrospinal fluid (CSF), has been extensively studied as diagnostic marker for Alzheimer's disease; however, results are variable. We investigated whether Abeta1-42 detection in CSF may be affected by handling temperature after lumbar puncture. CSF was collected from patients affected by probable AD (n=27), other dementias (OD) (n=24), or other neurological disorders without cognitive impairment (OND) (n=23). After lumbar puncture, CSF samples were either maintained at 37 degrees C, or handled according to standard procedures and centrifuged at 4 degrees C for 10 min; thereafter, one aliquot was further stored at 4 degrees C and another at 37 degrees C, before freezing all samples 90 min later at -80 degrees C, pending analysis. Abeta1-42 and total tau were determined using a commercially available sandwich enzyme-linked immunosorbent assay ELISA. Reduced Abeta1-42 and increased total tau CSF levels were confirmed as characteristic hallmarks of the OD and AD groups, providing standard measurement in samples stored at 4 degrees C before freezing. However, avoiding cooling or reheating CSF from 4 to 37 degrees C before freezing strikingly increased the Abeta1-42 concentration detectable in the AD group (P<0.01), but not in control groups. The results indicate that a pool of Abeta1-42 cannot be detectable in the CSF of AD patients, because standard preanalytical cooling masks in some ways the epitope recognized by Abeta1-42 specific antibodies. Moreover, our study suggests that low temperature could induce Abeta1-42 conformational changes and multimeric aggregates in probable AD, but, more importantly, Abeta1-42 aggregation could be reversible.
Sancesario, G.M., Esposito, Z., Nuccetelli, M.S., Sorge, R.P., Martorana, A., Federici, G., et al. (2010). Abeta1-42 Detection in CSF of Alzheimer's disease is influenced by handling temperature: indication of reversible Abeta1-42 aggregation?. EXPERIMENTAL NEUROLOGY, 223(2), 371-376.
|Tipologia:||Articolo su rivista|
|Citazione:||Sancesario, G.M., Esposito, Z., Nuccetelli, M.S., Sorge, R.P., Martorana, A., Federici, G., et al. (2010). Abeta1-42 Detection in CSF of Alzheimer's disease is influenced by handling temperature: indication of reversible Abeta1-42 aggregation?. EXPERIMENTAL NEUROLOGY, 223(2), 371-376.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.expneurol.2009.07.028|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||giu-2010|
|Titolo:||Abeta1-42 Detection in CSF of Alzheimer's disease is influenced by handling temperature: indication of reversible Abeta1-42 aggregation?|
|Autori:||Sancesario, GM; Esposito, Z; Nuccetelli, MS; Sorge, RP; Martorana, A; Federici, G; Bernardi, G; Bernardini, S; Sancesario, G|
|Appare nelle tipologie:||01 - Articolo su rivista|