The HIV-1 coat protein, gp120 (100 ng given intracerebroventricularly (i.c.v.) daily for seven consecutive days) causes DNA fragmentation in the brain neocortex of rat. In neocortical cells bearing ultrastructural features typical of apoptosis, electron microscopy revealed specific immunopositivity for neurofilament cytoskeletal proteins, suggesting the neuronal nature of dying cells. Neuronal apoptosis by gp120 implicates CXCR4 chemokine receptors; in fact, in rats receiving a single daily, non-neurotoxic, dose of SDF-1alpha (0.25 pmoles given i.c.v. for 7 days before gp120), the natural ligand of CXCR4 receptor, apoptosis was significantly hindered. The mechanism of SDF-1alpha protection involves inhibition of gp120-enhanced expression of IL-1beta, a cytokine implicated in the mechanisms of apoptosis induced by the viral protein in the neocortex of rat.

Corasaniti, M., PICCIRILLI, S., Paoletti, A., NISTICO', R.G., Stringaro, A., Malorni, W., et al. (2001). Evidence that the HIV-1 coat protein gp120 causes neuronal apoptosis in the neocortex of rat via a mechanism involving CXCR4 chemokine receptor. NEUROSCIENCE LETTERS, 312(2), 67-70.

Evidence that the HIV-1 coat protein gp120 causes neuronal apoptosis in the neocortex of rat via a mechanism involving CXCR4 chemokine receptor

PICCIRILLI, SILVIA;NISTICO', ROBERT GIOVANNI;FINAZZI AGRO', ALESSANDRO;BAGETTA, GIACINTO
2001

Abstract

The HIV-1 coat protein, gp120 (100 ng given intracerebroventricularly (i.c.v.) daily for seven consecutive days) causes DNA fragmentation in the brain neocortex of rat. In neocortical cells bearing ultrastructural features typical of apoptosis, electron microscopy revealed specific immunopositivity for neurofilament cytoskeletal proteins, suggesting the neuronal nature of dying cells. Neuronal apoptosis by gp120 implicates CXCR4 chemokine receptors; in fact, in rats receiving a single daily, non-neurotoxic, dose of SDF-1alpha (0.25 pmoles given i.c.v. for 7 days before gp120), the natural ligand of CXCR4 receptor, apoptosis was significantly hindered. The mechanism of SDF-1alpha protection involves inhibition of gp120-enhanced expression of IL-1beta, a cytokine implicated in the mechanisms of apoptosis induced by the viral protein in the neocortex of rat.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/14
English
AIDS Dementia Complex; Animals; Apoptosis; Chemokine CXCL12; Chemokines, CXC; Dose-Response Relationship, Drug; Glial Fibrillary Acidic Protein; HIV Envelope Protein gp120; HIV-1; Immunohistochemistry; In Situ Nick-End Labeling; Interleukin-1; Male; Microglia; Microscopy, Electron; Neocortex; Nerve Degeneration; Neurofilament Proteins; Neurons; Neurotoxins; Rats; Rats, Wistar; Receptors, CXCR4
Corasaniti, M., PICCIRILLI, S., Paoletti, A., NISTICO', R.G., Stringaro, A., Malorni, W., et al. (2001). Evidence that the HIV-1 coat protein gp120 causes neuronal apoptosis in the neocortex of rat via a mechanism involving CXCR4 chemokine receptor. NEUROSCIENCE LETTERS, 312(2), 67-70.
Corasaniti, M; Piccirilli, S; Paoletti, A; Nistico', Rg; Stringaro, A; Malorni, W; FINAZZI AGRO', A; Bagetta, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/133247
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