Apoptosis of neurons and astrocytes has been found in patients undergoing AIDS dementia complex. We demonstrated that supernatants from human primary macrophages (M/M) infected by HIV-1 lead human astroglial cells to oxidative stress, as shown by elevated levels of malondialdehyde, and then to apoptosis. Electron microscopy of astrocytes shortly incubated with HIV-1-infected M/M supernatants showed apoptotic blebbing, cytoplasmic loss, and chromatin condensation. Apoptosis was antagonized by pretreating astrocytes with the nonpeptidic superoxide dismutase (SOD) mimetic M40401 but not with anti-HIV-1 compounds, thus showing that apoptosis of astrocytes driven by HIV-1-infected M/M supernatants is mainly mediated by abnormal production of superoxide anions without relationship to HIV-1 replication in such cells. Overall results support the role of oxidative stress mediated by HIV-1-infected M/M as one of the leading causes of neurodegeneration in patients with HIV-1 and suggest the use of nonpeptidic SOD mimetics to counteract HIV-1-related neurological disorders.

MOLLACE, V., Salvemini, D., Riley, D., Muscoli, C., Iannone, M., Granato, T., et al. (2002). The contribution of oxidative stress in apoptosis of human-cultured astroglial cells induced by supernatants of HIV-1-infected macrophages. JOURNAL OF LEUKOCYTE BIOLOGY, 71(1), 65-72.

The contribution of oxidative stress in apoptosis of human-cultured astroglial cells induced by supernatants of HIV-1-infected macrophages

MOLLACE, VINCENZO;MODESTI, ANDREA;NISTICO', ROBERT GIOVANNI;BERTOLI, ADA;PERNO, CARLO FEDERICO;AQUARO, STEFANO
2002-01

Abstract

Apoptosis of neurons and astrocytes has been found in patients undergoing AIDS dementia complex. We demonstrated that supernatants from human primary macrophages (M/M) infected by HIV-1 lead human astroglial cells to oxidative stress, as shown by elevated levels of malondialdehyde, and then to apoptosis. Electron microscopy of astrocytes shortly incubated with HIV-1-infected M/M supernatants showed apoptotic blebbing, cytoplasmic loss, and chromatin condensation. Apoptosis was antagonized by pretreating astrocytes with the nonpeptidic superoxide dismutase (SOD) mimetic M40401 but not with anti-HIV-1 compounds, thus showing that apoptosis of astrocytes driven by HIV-1-infected M/M supernatants is mainly mediated by abnormal production of superoxide anions without relationship to HIV-1 replication in such cells. Overall results support the role of oxidative stress mediated by HIV-1-infected M/M as one of the leading causes of neurodegeneration in patients with HIV-1 and suggest the use of nonpeptidic SOD mimetics to counteract HIV-1-related neurological disorders.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/14
English
Apoptosis; Astrocytes; Cells, Cultured; Culture Media, Conditioned; HIV Infections; Humans; Macrophages; Oxidative Stress; HIV-1
MOLLACE, V., Salvemini, D., Riley, D., Muscoli, C., Iannone, M., Granato, T., et al. (2002). The contribution of oxidative stress in apoptosis of human-cultured astroglial cells induced by supernatants of HIV-1-infected macrophages. JOURNAL OF LEUKOCYTE BIOLOGY, 71(1), 65-72.
Mollace, V; Salvemini, D; Riley, D; Muscoli, C; Iannone, M; Granato, T; Masuelli, L; Modesti, A; Rotiroti, D; Nistico', Rg; Bertoli, A; Perno, Cf; Aquaro, S
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Descrizione: The contribution of oxidative stress in apoptosis of human-cultured astroglial cells induced by supernatants of HIV-1-infected macrophages
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/133238
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