The effect of cocaine on brain regional metabolism of L-arginine to nitric oxide (NO) has been studied in rat by measuring the level of citrulline, the co-product of NO synthesis, using a HPLC based methodology. A single i.p. administration of 1 mg/kg cocaine, and a daily treatment for up to 5 consecutive days, failed to affect significantly citrulline content in the striatum, hippocampus and cortex. By contrast, in these regions of the brain a single or 5-day repeated higher dose of cocaine (10 mg/kg, i.p.) caused a significant increase in the co-product of NO synthesis and this has been abolished in a stereoselective fashion by L-NAME (10 mg/kg i.p. given 30 min before). Under cocaine high dose treatment, 1 h acoustic stimulation, which per se resulted ineffective, enhanced stimulant-induced increases in citrulline content seen in the striatum and abolished the increase of this amino acid observed in the hippocampus and cortex both after single or 5-day repeated injection of cocaine. In conclusion, these data demonstrate that cocaine stimulates the conversion of L-arginine to NO in the brain of rat and this is affected by concomitant exposure to acoustic stimulation.

Bagetta, G., Rodinò, P., Arabia, A., Massoud, R., Paoletti, A., Nistico', R.g., et al. (1999). Systemic administration of cocaine, given alone or in combination with sensory stimuli, differentially affects L-arginine-nitric oxide metabolism in discrete regions of the brain of rat. NEUROSCIENCE LETTERS, 266(3), 153-156 [10.1016/S0304-3940(99)00290-6].

Systemic administration of cocaine, given alone or in combination with sensory stimuli, differentially affects L-arginine-nitric oxide metabolism in discrete regions of the brain of rat

BAGETTA, GIACINTO;MASSOUD, RENATO;NISTICO', ROBERT GIOVANNI;
1999-05-14

Abstract

The effect of cocaine on brain regional metabolism of L-arginine to nitric oxide (NO) has been studied in rat by measuring the level of citrulline, the co-product of NO synthesis, using a HPLC based methodology. A single i.p. administration of 1 mg/kg cocaine, and a daily treatment for up to 5 consecutive days, failed to affect significantly citrulline content in the striatum, hippocampus and cortex. By contrast, in these regions of the brain a single or 5-day repeated higher dose of cocaine (10 mg/kg, i.p.) caused a significant increase in the co-product of NO synthesis and this has been abolished in a stereoselective fashion by L-NAME (10 mg/kg i.p. given 30 min before). Under cocaine high dose treatment, 1 h acoustic stimulation, which per se resulted ineffective, enhanced stimulant-induced increases in citrulline content seen in the striatum and abolished the increase of this amino acid observed in the hippocampus and cortex both after single or 5-day repeated injection of cocaine. In conclusion, these data demonstrate that cocaine stimulates the conversion of L-arginine to NO in the brain of rat and this is affected by concomitant exposure to acoustic stimulation.
14-mag-1999
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/14 - FARMACOLOGIA
English
Acoustic Stimulation; Animals; Arginine; Brain; Central Nervous System Stimulants; Chromatography, High Pressure Liquid; Citrulline; Cocaine; Corpus Striatum; Drug Administration Schedule; Frontal Lobe; Hippocampus; Injections, Intraperitoneal; Male; Nitric Oxide; Rats; Rats, Wistar; Brain Mapping
Bagetta, G., Rodinò, P., Arabia, A., Massoud, R., Paoletti, A., Nistico', R.g., et al. (1999). Systemic administration of cocaine, given alone or in combination with sensory stimuli, differentially affects L-arginine-nitric oxide metabolism in discrete regions of the brain of rat. NEUROSCIENCE LETTERS, 266(3), 153-156 [10.1016/S0304-3940(99)00290-6].
Bagetta, G; Rodinò, P; Arabia, A; Massoud, R; Paoletti, A; Nistico', Rg; Passantino, L; Preziosi, P
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Descrizione: Systemic administration of cocaine, given alone or in combination with sensory stimuli, differentially affects L-arginine-nitric oxide metabolism in discrete regions of the brain of rat
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/133056
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