The effect of subchronic intracerebroventricular (i.c.v.) injection of the human immunodeficiency virus type 1 (HIV-1) recombinant protein gp120 (100 ng, given daily for up to 7 consecutive days) on cyclooxygenase type 2 (COX-2) expression was studied by immunohistochemistry in the brain of adult rats. In comparison to control, bovine serum albumin (100 ng, given i.c.v. for up to 7 days) treated animals (n = 6), a single daily injection of the viral protein for 7 consecutive days enhanced the number of COX-2 immunoreactive cells in the brain cortex of rats (n = 6 per group) and this was accompanied by a 50% increase over control PGE2 content in whole brain tissue homogenates (n = 6). In another series of experiments, pretreatment of rats (n = 6) with indomethacin (6.0 mg/kg given i.p. 1 h before gp120 injection), an inhibitor COX activity, prevented apoptotic death typically produced by gp120 in the neocortex of rat suggesting that enhancement of COX-2 expression may be involved in the mechanisms of apoptosis yielded by the HIV-1 coat protein.

Bagetta, G., Corasaniti, M., Paoletti, A., Berliocchi, L., NISTICO', R.G., Giammarioli, A., et al. (1998). HIV-1 gp120-induced apoptosis in the rat neocortex involves enhanced expression of cyclo-oxygenase type 2 (COX-2). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 244(3), 819-824 [10.1006/bbrc.1998.8321].

HIV-1 gp120-induced apoptosis in the rat neocortex involves enhanced expression of cyclo-oxygenase type 2 (COX-2)

NISTICO', ROBERT GIOVANNI;
1998

Abstract

The effect of subchronic intracerebroventricular (i.c.v.) injection of the human immunodeficiency virus type 1 (HIV-1) recombinant protein gp120 (100 ng, given daily for up to 7 consecutive days) on cyclooxygenase type 2 (COX-2) expression was studied by immunohistochemistry in the brain of adult rats. In comparison to control, bovine serum albumin (100 ng, given i.c.v. for up to 7 days) treated animals (n = 6), a single daily injection of the viral protein for 7 consecutive days enhanced the number of COX-2 immunoreactive cells in the brain cortex of rats (n = 6 per group) and this was accompanied by a 50% increase over control PGE2 content in whole brain tissue homogenates (n = 6). In another series of experiments, pretreatment of rats (n = 6) with indomethacin (6.0 mg/kg given i.p. 1 h before gp120 injection), an inhibitor COX activity, prevented apoptotic death typically produced by gp120 in the neocortex of rat suggesting that enhancement of COX-2 expression may be involved in the mechanisms of apoptosis yielded by the HIV-1 coat protein.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/14
English
Animals; Cyclooxygenase 2; Cyclooxygenase 2 Inhibitors; Cyclooxygenase Inhibitors; DNA Fragmentation; Dinoprostone; HIV Envelope Protein gp120; Indomethacin; Injections, Intraventricular; Isoenzymes; Male; Neocortex; Prostaglandin-Endoperoxide Synthases; Rats; Rats, Wistar; Apoptosis; HIV-1
Bagetta, G., Corasaniti, M., Paoletti, A., Berliocchi, L., NISTICO', R.G., Giammarioli, A., et al. (1998). HIV-1 gp120-induced apoptosis in the rat neocortex involves enhanced expression of cyclo-oxygenase type 2 (COX-2). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 244(3), 819-824 [10.1006/bbrc.1998.8321].
Bagetta, G; Corasaniti, M; Paoletti, A; Berliocchi, L; Nistico', Rg; Giammarioli, A; Malorni, W; Finazzi Agro, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/133008
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