The secondary structure of ordered peptides in solution usually exhibits helical distortions that are enhanced by the shortness of the backbone chain. These distortions are reflected in the optical properties of amide NH infrared absorption and in the shape of the CD spectra. These effects are exemplified here in a series of short, intramolecularly H-bonded peptides of the general formula F[(αMe)Val]r-T-[(αMe)Val]2NHt-Bu where T = Toac and F = Fmoc, a nitroxide-based α-amino acid quencher and a fluorophoric Nα protecting group, respectively, where r = 0−3. Their structural features in methanol were determined by a combined approach of time-resolved fluorescence resonance energy transfer measurements and molecular mechanics calculations. They show a rather compact arrangement of the probes around the distorted, 310-helical backbone chain, which accounts for the slow interconversion between conformational substates on the nanosecond time scale.

Pispisa, B., Palleschi, A., Stella, L., Venanzi, M., Mazzuca, C., Formaggio, F., et al. (2002). Effects of helical distortions on the optical properties of amide NH infrared absorption in short peptides in solution. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, 106(22), 5733-5738 [10.1021/jp012599a].

Effects of helical distortions on the optical properties of amide NH infrared absorption in short peptides in solution

PISPISA, BASILIO;PALLESCHI, ANTONIO;STELLA, LORENZO;VENANZI, MARIANO;MAZZUCA, CLAUDIA;
2002

Abstract

The secondary structure of ordered peptides in solution usually exhibits helical distortions that are enhanced by the shortness of the backbone chain. These distortions are reflected in the optical properties of amide NH infrared absorption and in the shape of the CD spectra. These effects are exemplified here in a series of short, intramolecularly H-bonded peptides of the general formula F[(αMe)Val]r-T-[(αMe)Val]2NHt-Bu where T = Toac and F = Fmoc, a nitroxide-based α-amino acid quencher and a fluorophoric Nα protecting group, respectively, where r = 0−3. Their structural features in methanol were determined by a combined approach of time-resolved fluorescence resonance energy transfer measurements and molecular mechanics calculations. They show a rather compact arrangement of the probes around the distorted, 310-helical backbone chain, which accounts for the slow interconversion between conformational substates on the nanosecond time scale.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/02 - Chimica Fisica
English
Pispisa, B., Palleschi, A., Stella, L., Venanzi, M., Mazzuca, C., Formaggio, F., et al. (2002). Effects of helical distortions on the optical properties of amide NH infrared absorption in short peptides in solution. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, 106(22), 5733-5738 [10.1021/jp012599a].
Pispisa, B; Palleschi, A; Stella, L; Venanzi, M; Mazzuca, C; Formaggio, F; Toniolo, C; Broxterman, Q
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/132975
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