During embryonic development, hair follicles (HFs) develop from an epidermal-mesenchymal cross talk between the ectoderm progenitor layer and the underlying dermis. Epidermal stem cell activation represents a crucial point both for HF morphogenesis and for hair regeneration. miR-24 is an anti-proliferative microRNA (miRNA), which is induced during differentiation of several cellular systems including the epidermis. Here, we show that miR-24 is expressed in the HF and has a role in hair morphogenesis. We generated transgenic mice ectopically expressing miR-24 under the K5 promoter. The K5::miR-24 animals display a marked defect in HF morphogenesis, with thinning of hair coat and altered HF structure. Expression of miR-24 alters the normal process of hair keratinocyte differentiation, leading to altered expression of differentiation markers. MiR-24 directly represses the hair keratinocyte stemness regulator Tcf-3. These results support the notion that microRNAs, and among them miR-24, have an important role in postnatal epidermal homeostasis.

Amelio, I., Lena, A.m., Bonanno, E., Melino, G., & Candi, E. (2013). miR-24 affects hair follicle morphogenesis targeting Tcf-3. CELL DEATH & DISEASE, 4(11), e922 [10.1038/cddis.2013.426].

miR-24 affects hair follicle morphogenesis targeting Tcf-3

Amelio, I;LENA, ANNA MARIA;BONANNO, ELENA;MELINO, GENNARO;CANDI, ELEONORA
2013

Abstract

During embryonic development, hair follicles (HFs) develop from an epidermal-mesenchymal cross talk between the ectoderm progenitor layer and the underlying dermis. Epidermal stem cell activation represents a crucial point both for HF morphogenesis and for hair regeneration. miR-24 is an anti-proliferative microRNA (miRNA), which is induced during differentiation of several cellular systems including the epidermis. Here, we show that miR-24 is expressed in the HF and has a role in hair morphogenesis. We generated transgenic mice ectopically expressing miR-24 under the K5 promoter. The K5::miR-24 animals display a marked defect in HF morphogenesis, with thinning of hair coat and altered HF structure. Expression of miR-24 alters the normal process of hair keratinocyte differentiation, leading to altered expression of differentiation markers. MiR-24 directly represses the hair keratinocyte stemness regulator Tcf-3. These results support the notion that microRNAs, and among them miR-24, have an important role in postnatal epidermal homeostasis.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10
Settore MED/08 - Anatomia Patologica
English
Animals; Blotting, Western; Cell Proliferation; Cells, Cultured; Fluorescent Antibody Technique; Hair Follicle; In Situ Hybridization; Mice; Mice, Transgenic; MicroRNAs; Morphogenesis; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factor 7-Like 1 Protein
Amelio, I., Lena, A.m., Bonanno, E., Melino, G., & Candi, E. (2013). miR-24 affects hair follicle morphogenesis targeting Tcf-3. CELL DEATH & DISEASE, 4(11), e922 [10.1038/cddis.2013.426].
Amelio, I; Lena, Am; Bonanno, E; Melino, G; Candi, E
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/131477
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