A hybrid assay, based on the properties of the lambda repressor, was developed to detect FtsZ dimerization in Escherichia coli in vivo. A gene fusion comprising the N-terminal end of the lambda cI repressor gene and the complete E. coli ftsZ gene was constructed. The fused protein resulted in a functional lambda repressor and was able to complement the thermosensitive mutant ftsZ84. Using the same strategy, a series of 10 novel mutants of FtsZ that are unable to dimerize was selected, and a deletion analysis of the protein was carried out. Characterization of these mutants allowed the identification of three separate FtsZ portions: the N-terminal of about 150 amino acids; the C-terminal of about 60 amino acids, which corresponds to the less conserved portion of the protein; and a central region of about 150 residues. Mutants belonging to this region would define the dimerization domain of FtsZ.
Di Lallo, G., Anderluzzi, D., Ghelardini, P., & Paolozzi, L. (1999). FtsZ dimerization in vivo. MOLECULAR MICROBIOLOGY, 32(2), 265-274.
Tipologia: | Articolo su rivista |
Citazione: | Di Lallo, G., Anderluzzi, D., Ghelardini, P., & Paolozzi, L. (1999). FtsZ dimerization in vivo. MOLECULAR MICROBIOLOGY, 32(2), 265-274. |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/19 - Microbiologia Generale |
Revisione (peer review): | Esperti anonimi |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1046/j.1365-2958.1999.01344.x |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | apr-1999 |
Titolo: | FtsZ dimerization in vivo |
Autori: | |
Autori: | Di Lallo, G; Anderluzzi, D; Ghelardini, P; Paolozzi, L |
Appare nelle tipologie: | 01 - Articolo su rivista |