Mitochondrial DNA from the yeast Kluyveromyces marxianus var. lactis (K.lactis) is a circular molecule of 39 kilobase-pairs. A genetic and physical map was constructed. We found that this genome contained a large number of guanine-cytosine (GC)-rich sequence clusters, many of which are characterized by the presence of SacII restriction sites (CCGCGG). The primary sequence of the GC clusters often showed a palindromic structure. These GC clusters were present in several varieties of K.marxianus, but not in others. The presence of these clusters is a major feature that distinguishes K.lactis strains from those of K.marxianus var. marxianus (including K.fragilis).
Ragnini, A., Fukuhara, H. (1988). Mitochondrial DNA of the yeast Kluyveromyces: guanine-cytosine rich sequence clusters. NUCLEIC ACIDS RESEARCH, 16(17), 8433-8442 [10.1093/nar/16.17.8433].
Mitochondrial DNA of the yeast Kluyveromyces: guanine-cytosine rich sequence clusters
RAGNINI, ANTONELLA;
1988-09-12
Abstract
Mitochondrial DNA from the yeast Kluyveromyces marxianus var. lactis (K.lactis) is a circular molecule of 39 kilobase-pairs. A genetic and physical map was constructed. We found that this genome contained a large number of guanine-cytosine (GC)-rich sequence clusters, many of which are characterized by the presence of SacII restriction sites (CCGCGG). The primary sequence of the GC clusters often showed a palindromic structure. These GC clusters were present in several varieties of K.marxianus, but not in others. The presence of these clusters is a major feature that distinguishes K.lactis strains from those of K.marxianus var. marxianus (including K.fragilis).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.