Binding and fate of the nerve growth factor (NGF) in pheochromocytoma cells (clone PC12) have been measured with the use of iodine-labeled ligand and with 125I-NGF antibodies. With such double approach it is possible to distinguish between surface bound and total NGF bound to PC12 cells. It is found that NGF-receptor complexes undergo down-regulation. This process is noticeable at low but not at high cell densities, and only in PC12 cells never exposed to NGF. Previous incubation with growth factor leads to the disappearance of down-regulation of NGF-receptor complexes. Assuming that this process is an indirect measure of NGF-receptor internalization, it is concluded that it is modulated by the cell density or by previous exposure to the factor. These findings are postulated to be relevant to the mechanism of action of NGF and to its multiple effects on target cells.

Biocca, S., Levi, A., Calissano, P. (1980). Cell density modulates receptor-mediated internalization of nerve growth factor in pheochromocytoma cells. JOURNAL OF RECEPTOR RESEARCH, 1(3), 373-387.

Cell density modulates receptor-mediated internalization of nerve growth factor in pheochromocytoma cells

BIOCCA, SILVIA;CALISSANO, PIETRO
1980-01-01

Abstract

Binding and fate of the nerve growth factor (NGF) in pheochromocytoma cells (clone PC12) have been measured with the use of iodine-labeled ligand and with 125I-NGF antibodies. With such double approach it is possible to distinguish between surface bound and total NGF bound to PC12 cells. It is found that NGF-receptor complexes undergo down-regulation. This process is noticeable at low but not at high cell densities, and only in PC12 cells never exposed to NGF. Previous incubation with growth factor leads to the disappearance of down-regulation of NGF-receptor complexes. Assuming that this process is an indirect measure of NGF-receptor internalization, it is concluded that it is modulated by the cell density or by previous exposure to the factor. These findings are postulated to be relevant to the mechanism of action of NGF and to its multiple effects on target cells.
1980
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
Con Impact Factor ISI
Adrenal Gland Neoplasms; Animals; Cell Count; Clone Cells; Hydrogen-Ion Concentration; Nerve Growth Factors; Pheochromocytoma; Receptors, Cell Surface; Receptors, Nerve Growth Factor; Time Factors
Biocca, S., Levi, A., Calissano, P. (1980). Cell density modulates receptor-mediated internalization of nerve growth factor in pheochromocytoma cells. JOURNAL OF RECEPTOR RESEARCH, 1(3), 373-387.
Biocca, S; Levi, A; Calissano, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/128147
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