In the preceding article [1] we identified the 34 kD single-stranded DNA-binding (ssb) protein, whose synthesis is inhibited in PC12 cells concomitantly with nerve growth factor (NGF)-induced mitotic arrest, with the enzyme lactic dehydrogenase (LDH-ssb protein). Localization studies performed with antibodies raised against the LDH-ssb protein demonstrate the presence of a pool of this protein in the nucleus of several cell types. The nuclear association of this protein is sensitive to DNase treatment of the cells and quantitative electron microscopy confirms that the LDH-ssb protein is located close to chromatin structures. These results point to a possible involvement of the LDH-ssb protein in some nuclear function(s).

Cattaneo, A., Biocca, S., Corvaja, N., Calissano, P. (1985). Nuclear localization of a lactic dehydrogenase with single-stranded DNA-binding properties. EXPERIMENTAL CELL RESEARCH, 161(1), 130-140.

Nuclear localization of a lactic dehydrogenase with single-stranded DNA-binding properties

BIOCCA, SILVIA;CALISSANO, PIETRO
1985-11

Abstract

In the preceding article [1] we identified the 34 kD single-stranded DNA-binding (ssb) protein, whose synthesis is inhibited in PC12 cells concomitantly with nerve growth factor (NGF)-induced mitotic arrest, with the enzyme lactic dehydrogenase (LDH-ssb protein). Localization studies performed with antibodies raised against the LDH-ssb protein demonstrate the presence of a pool of this protein in the nucleus of several cell types. The nuclear association of this protein is sensitive to DNase treatment of the cells and quantitative electron microscopy confirms that the LDH-ssb protein is located close to chromatin structures. These results point to a possible involvement of the LDH-ssb protein in some nuclear function(s).
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/12
English
Con Impact Factor ISI
Animals; Astrocytes; Cell Line; Cell Nucleus; Chromatin; Cytoplasm; DNA, Single-Stranded; DNA-Binding Proteins; Deoxyribonucleases; Fluorescent Antibody Technique; L-Lactate Dehydrogenase; Microscopy, Electron; Pheochromocytoma; Quail; Rats; Ribonucleases
Cattaneo, A., Biocca, S., Corvaja, N., Calissano, P. (1985). Nuclear localization of a lactic dehydrogenase with single-stranded DNA-binding properties. EXPERIMENTAL CELL RESEARCH, 161(1), 130-140.
Cattaneo, A; Biocca, S; Corvaja, N; Calissano, P
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/128112
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