Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development.

Benvenuto, E., Ordàs, R., Tavazza, R., Ancora, G., Biocca, S., Cattaneo, A., et al. (1991). 'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants. PLANT MOLECULAR BIOLOGY, 17(4), 865-874.

'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants

BIOCCA, SILVIA;
1991-10

Abstract

Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/12
Settore BIO/13
English
Con Impact Factor ISI
Agrobacterium tumefaciens; Blotting, Northern; Blotting, Western; Escherichia coli; Gene Expression; Genetic Vectors; Immunoglobulin Heavy Chains; Immunoglobulin Variable Region; Microscopy, Fluorescence; Plants, Genetically Modified; Plasmids; Recombinant Fusion Proteins; Substance P; Tobacco; Transformation, Genetic; Plants, Toxic
Benvenuto, E., Ordàs, R., Tavazza, R., Ancora, G., Biocca, S., Cattaneo, A., et al. (1991). 'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants. PLANT MOLECULAR BIOLOGY, 17(4), 865-874.
Benvenuto, E; Ordàs, R; Tavazza, R; Ancora, G; Biocca, S; Cattaneo, A; Galeffi, P
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/128070
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