The intracellular expression of antibodies in mammalian cells is a strategy to inhibit the in vivo function of selected molecules but is limited by the unpredictable behaviour of antibodies when intracellularly expressed. Recent advances in the field of antibody expression in Escherichia coli show that the introduction of mutations can improve the properties of some antibody domains, but the general applicability of this approach to intracellular antibodies remains to be proved. As a complement to rational approaches, we describe selection schemes in which antibodies are selected on the basis of their performance in vivo as intracellular antibodies.

Cattaneo, A., Biocca, S. (1999). The selection of intracellular antibodies. TRENDS IN BIOTECHNOLOGY, 17(3), 115-121.

The selection of intracellular antibodies

BIOCCA, SILVIA
1999-03-01

Abstract

The intracellular expression of antibodies in mammalian cells is a strategy to inhibit the in vivo function of selected molecules but is limited by the unpredictable behaviour of antibodies when intracellularly expressed. Recent advances in the field of antibody expression in Escherichia coli show that the introduction of mutations can improve the properties of some antibody domains, but the general applicability of this approach to intracellular antibodies remains to be proved. As a complement to rational approaches, we describe selection schemes in which antibodies are selected on the basis of their performance in vivo as intracellular antibodies.
mar-1999
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
Con Impact Factor ISI
Animals; Antibodies, Bacterial; Biotechnology; COS Cells; Escherichia coli; Immunophenotyping
Cattaneo, A., Biocca, S. (1999). The selection of intracellular antibodies. TRENDS IN BIOTECHNOLOGY, 17(3), 115-121.
Cattaneo, A; Biocca, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/127595
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