Acanthamoeba keratitis is a rare but serious corneal disease, often observed in contact lens wearers. Clinical treatment of infected patients frequently involves the use of polyhexamethylene biguanide (PHMB), a polymer used as a disinfectant and antiseptic, which is toxic also for the epithelial cells of the cornea. Prompt and effective diagnostic tools are hence highly desiderable for both starting early therapy and timely suspension of the treatment. In this work we use Raman microspectroscopy to analyse in vitro a single Acanthamoeba cell in cystic phase. In particular, we investigate the effect of PHMB at the single-cell level, providing useful information on both the underlying biochemical mechanism and the time frame for Acanthamoeba eradication in ocular infections. Furthermore, we demonstrate that Raman spectroscopy, in conjunction with standard multivariate analysis methods, allows discriminating between live and dead Acanthamoebas, which is fundamental to optimizing patients' treatment.

Rusciano, G., Capriglione, P., Pesce, G., Del Prete, S., Cennamo, G., DI CAVE, D., et al. (2013). Raman Microspectroscopy Analysis in the Treatment of Acanthamoeba Keratitis. PLOS ONE, 8(8), e72127 [10.1371/journal.pone.0072127].

Raman Microspectroscopy Analysis in the Treatment of Acanthamoeba Keratitis

DI CAVE, DAVID;
2013-01-01

Abstract

Acanthamoeba keratitis is a rare but serious corneal disease, often observed in contact lens wearers. Clinical treatment of infected patients frequently involves the use of polyhexamethylene biguanide (PHMB), a polymer used as a disinfectant and antiseptic, which is toxic also for the epithelial cells of the cornea. Prompt and effective diagnostic tools are hence highly desiderable for both starting early therapy and timely suspension of the treatment. In this work we use Raman microspectroscopy to analyse in vitro a single Acanthamoeba cell in cystic phase. In particular, we investigate the effect of PHMB at the single-cell level, providing useful information on both the underlying biochemical mechanism and the time frame for Acanthamoeba eradication in ocular infections. Furthermore, we demonstrate that Raman spectroscopy, in conjunction with standard multivariate analysis methods, allows discriminating between live and dead Acanthamoebas, which is fundamental to optimizing patients' treatment.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
English
Acanthamoeba; Acanthamoeba Keratitis; Amebicides; Biguanides; Contact Lenses; Cytoplasm; Humans; Models, Biological; Multivariate Analysis; Principal Component Analysis; Single-Cell Analysis; Spectrum Analysis, Raman
Rusciano, G., Capriglione, P., Pesce, G., Del Prete, S., Cennamo, G., DI CAVE, D., et al. (2013). Raman Microspectroscopy Analysis in the Treatment of Acanthamoeba Keratitis. PLOS ONE, 8(8), e72127 [10.1371/journal.pone.0072127].
Rusciano, G; Capriglione, P; Pesce, G; Del Prete, S; Cennamo, G; DI CAVE, D; Cerulli, L; Sasso, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/127372
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