Aims: Classic virological tests are time consuming and labour-intensive; realtime RT-PCR has proven to be a fast method to detect and quantify enterovirus genomes in clinical and environmental samples. This method is unable to discriminate between infective and noninfective enterovirus particles; few clinical studies have compared real-time RT-PCR and viral culture. We wondered if the enterovirus genome quantification could be correlated to the infectivity. Methods and Results: We used the statistical approach to verify our hypotheses to correlate data, obtained by the standard method (most probable number of cytopathic units—MPNCU) and molecular test (real-time RT-PCR), on wastewater treatment plant samples. Chi-squared test was used, considering several cut-off values (‘50’-‘100’-‘200’ genome copy numbers), to determine statistical significance in comparison of the two methods. Chi-square value was not significant when cut-off of 50 (P = 0Æ103) and 100 (P = 0Æ178) was assumed but was significant with cut-off of 200 (P = 0Æ044). Conclusion: This limit, 200 genome copy, could be used as cut-off value to indicate enterovirus survival in environmental monitoring. Significant and Impact of the Study: To introduce a fast procedure that is able to compensate for disadvantages of cell culture method for viral environmental analyses.

Donia, D., Bonanni, E., Diaco, L., & Divizia, M. (2010). Statistical correlation between enterovirus genome copy numbers and infectious viral particles in wastewater samples. LETTERS IN APPLIED MICROBIOLOGY, 50(2), 237-240 [10.1111/j.1472-765X.2009.02775.x].

Statistical correlation between enterovirus genome copy numbers and infectious viral particles in wastewater samples

DONIA, DOMENICA TOMMASA;DIVIZIA, MAURIZIO
2010

Abstract

Aims: Classic virological tests are time consuming and labour-intensive; realtime RT-PCR has proven to be a fast method to detect and quantify enterovirus genomes in clinical and environmental samples. This method is unable to discriminate between infective and noninfective enterovirus particles; few clinical studies have compared real-time RT-PCR and viral culture. We wondered if the enterovirus genome quantification could be correlated to the infectivity. Methods and Results: We used the statistical approach to verify our hypotheses to correlate data, obtained by the standard method (most probable number of cytopathic units—MPNCU) and molecular test (real-time RT-PCR), on wastewater treatment plant samples. Chi-squared test was used, considering several cut-off values (‘50’-‘100’-‘200’ genome copy numbers), to determine statistical significance in comparison of the two methods. Chi-square value was not significant when cut-off of 50 (P = 0Æ103) and 100 (P = 0Æ178) was assumed but was significant with cut-off of 200 (P = 0Æ044). Conclusion: This limit, 200 genome copy, could be used as cut-off value to indicate enterovirus survival in environmental monitoring. Significant and Impact of the Study: To introduce a fast procedure that is able to compensate for disadvantages of cell culture method for viral environmental analyses.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/42 - Igiene Generale e Applicata
English
Con Impact Factor ISI
chi-squared, cut-off, genome copy number, infectivity, statistical analysis.
Donia, D., Bonanni, E., Diaco, L., & Divizia, M. (2010). Statistical correlation between enterovirus genome copy numbers and infectious viral particles in wastewater samples. LETTERS IN APPLIED MICROBIOLOGY, 50(2), 237-240 [10.1111/j.1472-765X.2009.02775.x].
Donia, Dt; Bonanni, E; Diaco, L; Divizia, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/12550
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