The influence of the excited-state lifetime of a fluorophore on its susceptibility to collisional quenching is discussed. In the case of cytochrome c, FRET to the heme moiety strongly reduces the tryptophan fluorescence lifetime. This should be taken into account to correctly evaluate the tryptophan solvent accessibility from acrylamide quenching experiments.
Stella, L. (2011). Fluorescence quenching, lifetimes, and fluorophore solvent accessibility. JOURNAL OF CHEMICAL EDUCATION, 88(6), 695-696 [10.1021/ed101147s].
Fluorescence quenching, lifetimes, and fluorophore solvent accessibility
STELLA, LORENZO
2011-01-01
Abstract
The influence of the excited-state lifetime of a fluorophore on its susceptibility to collisional quenching is discussed. In the case of cytochrome c, FRET to the heme moiety strongly reduces the tryptophan fluorescence lifetime. This should be taken into account to correctly evaluate the tryptophan solvent accessibility from acrylamide quenching experiments.File in questo prodotto:
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