In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.

Scaldaferri, M., Klinger, F.g., Farini, D., Di Carlo, A., Carsetti, R., Giorda, E., et al. (2015). Hematopoietic activity in putative mouse primordial germ cell populations. MECHANISMS OF DEVELOPMENT, 136, 53-63 [10.1016/j.mod.2015.02.002].

Hematopoietic activity in putative mouse primordial germ cell populations

KLINGER, FRANCESCA GIOIA;FARINI, DONATELLA;DE FELICI, MASSIMO
2015-01-01

Abstract

In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.
2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
English
Allantois; Germline; Hematopoiesis; Oct3/4; Primordial germ cells
Scaldaferri, M., Klinger, F.g., Farini, D., Di Carlo, A., Carsetti, R., Giorda, E., et al. (2015). Hematopoietic activity in putative mouse primordial germ cell populations. MECHANISMS OF DEVELOPMENT, 136, 53-63 [10.1016/j.mod.2015.02.002].
Scaldaferri, M; Klinger, Fg; Farini, D; Di Carlo, A; Carsetti, R; Giorda, E; DE FELICI, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/116451
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